Cell Counting kit-8

Absorbance

450 nm

Application

Cell Counting

Cat no : PF00004



Product Information

Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfobenzene)- 2H-tetrazole monosodium salt), is a rapid and highlysensitive detection kit widely used in cell proliferation and cytotoxicity.In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.

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WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST- 8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.

Product     

100T            

500T            

3000T            

6000T          

 cck-8            

liquid          

Storage

It is effective for 2 years at 4℃ and 3 years at -20℃.

Protocol

CCK8 Protocol

Calculation

Cell viability=[(As-Ab)/(Ac-Ab)]×100% 

Inhibition rate=[(Ac-As)/(Ac-Ab)]×100% 

As:The absorbance of the experiment hole (medium containing cells, CCK-8, substance to be measured) Ac:Absorbance of control hole (medium containing cells, CCK-8, no substance to be measured) 

Ab:Blank (medium without cells and substance to be measured, CCK-8) absorbance

Publications

ApplicationTitle

Exp Ther Med

Sinomenine activation of Nrf2 signaling prevents inflammation and cerebral injury in a mouse model of ischemic stroke.

Authors - Fangfang Bi

Appl Physiol Nutr Metab

Exercise improves lipid metabolism disorders induced by high-fat diet in a SESN2/JNK- independent manner.

Authors - Tianyi Wang

Pharm Biol

Kaempferol-induced GPER upregulation attenuates atherosclerosis via the PI3K/AKT/Nrf2 pathway.

Authors - Zhuo Feng

Biomed Mater

L-polylactic acid porous microspheres enhance the mechanical properties and in vivo stability of degummed silk/silk fibroin/gelatin scaffold.

Authors - Tian Li