Cell Counting kit-8
Cat no : PF00004
Validation Data Gallery
Product Information
Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfobenzene)- 2H-tetrazole monosodium salt), is a rapid and highlysensitive detection kit widely used in cell proliferation and cytotoxicity.In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.
WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST- 8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.
Product | 500T | 3000T |
cck-8 | liquid | |
Storage
It is effective for 2 years at 4℃ and 3 years at -20℃.
Protocol
Calculation
Cell viability=[(As-Ab)/(Ac-Ab)]×100%
Inhibition rate=[(Ac-As)/(Ac-Ab)]×100%
As:The absorbance of the experiment hole (medium containing cells, CCK-8, substance to be measured) Ac:Absorbance of control hole (medium containing cells, CCK-8, no substance to be measured)
Ab:Blank (medium without cells and substance to be measured, CCK-8) absorbance
Cited in Article as
PF00004, Cell Counting kit-8, Proteintech, IL, USA
Documentation
| SDS |
|---|
| Cell counting kit-8 SDS (EU) |
| Cell counting kit-8 SDS (US) |
Publications
| Application | Title |
|---|---|
Brief Bioinform Ferroptosis-related lncRNA pairs to predict the clinical outcome and molecular characteristics of pancreatic ductal adenocarcinoma. | |
Bioact Mater TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair. | |
Int J Biol Macromol Pinus massoniana pollen polysaccharides alleviate LPS-induced myocardial injury through p110β-mediated inhibition of the PI3K/AKT/NFκB pathway | |
Phytomedicine S. glabra exerts anti-lung cancer effects by inducing ferroptosis and anticancer immunity | |
J Transl Med ESRRG-PKM2 axis reprograms metabolism to suppress esophageal squamous carcinoma progression and enhance anti-PD-1 therapy efficacy | |
J Invest Dermatol Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models. |
