Product Information
Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfobenzene)- 2H-tetrazole monosodium salt), is a rapid and highlysensitive detection kit widely used in cell proliferation and cytotoxicity.In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.
WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST- 8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.
Product | 500T | 3000T |
cck-8 | liquid | |
Storage
It is effective for 2 years at 4℃ and 3 years at -20℃.
Protocol
Calculation
Cell viability=[(As-Ab)/(Ac-Ab)]×100%
Inhibition rate=[(Ac-As)/(Ac-Ab)]×100%
As:The absorbance of the experiment hole (medium containing cells, CCK-8, substance to be measured) Ac:Absorbance of control hole (medium containing cells, CCK-8, no substance to be measured)
Ab:Blank (medium without cells and substance to be measured, CCK-8) absorbance
Cited in Article as
PF00004, Cell Counting kit-8, Proteintech, IL, USA
Documentation
| SDS |
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| Cell Counting kit-8 Datasheet |
| Cell Counting kit-8 Datasheet |
| Datasheet |
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| Cell Counting kit-8 Datasheet |
Publications
| Application | Title |
|---|---|
Signal Transduct Target Ther Targeting N(1)-methyladenosine modification in osteoblasts through tRNA methyltransferase 10C reverses mitochondrial dysfunction and ameliorates osteoporosis. | |
Adv Mater A Metabo-Reprogramming Niche Remodeling System Halts Osteoarthritis by Restoring the FGF21-Arginine Axis. | |
Bioact Mater TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair. | |
J Extracell Vesicles Exosomal Oleic Acid Promotes Lymphangiogenesis and Nodal Metastasis in Cervical Cancer via the AKT/mTOR Pathway. | |
ACS Nano Ferritin-Based Mild Magnetic Hyperthermia Therapy for Bladder Cancer: A Synergistic Anti-Inflammatory Strategy. | |
ACS Nano Tumor Vaccine Exploiting Membranes with Influenza Virus-Induced Immunogenic Cell Death to Decorate Polylactic Coglycolic Acid Nanoparticles |
