Cleaved PARP1 (Asp214) Recombinant antibody, PBS Only

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  WB analysis of HeLa using 85175-5-RR (same clone as 85175-5-PBS)

  Staurosporine treated HeLa cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85175-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 85175-5-RR (same clone as 85175-5-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85175-5-RR (Cleaved PARP1 (Asp214) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85175-5-PBS in a different storage buffer formulation.

Cleaved PARP1 (Asp214) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85175-5-PBS

Clone No.243142B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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Product Information

85175-5-PBS targets Cleaved PARP1 (Asp214) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated Molecular Weight: 1014 aa, 113 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC037545
• Gene Symbol: PARP1
• Gene ID (NCBI): 142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09874
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.