CoraLite® Plus 488-Phalloidin (green)

Excitation Laser

490 nm

Emission

515 nm

Application

IF

Cat no : PF00001



Product Information

Phalloidin is a toxin isolated from mushrooms that binds F-Actin in the cytoskeleton. This product is conjugated to our fluorescent CoraLite(R) dye to stain F-actin for labeling, identifying and quantifying actin filaments in immunostaining studies. Compared to many fluorescent dyes, CoraLite(R) dyes have advantages in brightness, light stability and water solubility. Excitation/Emission maxima wavelengths are 490 nm/515 nm.

Conjugated

CoraLite® Plus 488-Phalloidin (green)

Ex/Em: 490/515 nm

Storage

Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. 

Protocol

Stock preparation: Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.

Fluorescence Microscopy Protocol

Cautions

This product is formulated as a freeze-dried powder. Please centrifuge it immediately before use and dissolve it in a suitable solvent before use. Phalloidin is highly toxic, please take protective measures while handling.

Stock preparation

Prepare a 200T/mL stock solution by dissolving lyophilized phalloidin (300T) in 1.5 mL sterile water and protect solution from light.

Cited in Article as

PF00001, CoraLite® Plus 488-Phalloidin (green), Proteintech, IL, USA

Publications

ApplicationTitle
IF

Acta Biomater

Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization

Authors - Yongzheng Li
IF

J Nanobiotechnology

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

Authors - Xiao Liu

Cancer Lett

CD63+ cancer-associated fibroblasts confer CDK4/6 inhibitor resistance to breast cancer cells by exosomal miR-20

Authors - Jiahui Sun

Bioact Mater

A novel mechanism of inhibiting in-stent restenosis with arsenic trioxide drug-eluting stent: Enhancing contractile phenotype of vascular smooth muscle cells via YAP pathway.

Authors - Yinping Zhao
IF

Otol Neurotol

Protective Effects of Gastrodin Against Gentamicin-Induced Vestibular Damage by the Notch Signaling Pathway

Authors - Wen Jiang

Biochem Pharmacol

Antiplatelet drug ticagrelor suppresses triple negative breast cancer metastasis by targeting PI3K

Authors - Rong Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Udesh (Verified Customer) (10-04-2023)

Worked well at 1:300 for 1H in 2% BSA at Room Temperature

  • Applications: Immunofluorescence
FH

Víctor (Verified Customer) (07-20-2023)

Really good antibody, at least in my staining of human fibroblasts. The intensity of the conjugated antibody is really good and it seems very specific binding microfilaments of the citoskeleton, with a very low background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human fibroblasts
FH

Niklas (Verified Customer) (11-08-2022)

great staining with even a high dilution factor

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: human fibroblasts