CoraLite®594 TUNEL Assay Apoptosis Detection Kit

Excitation Laser

593 nm

Emission

614 nm

Application

FC, IF

Publications

4

Cat no : PF00009

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Validation Data Gallery

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  The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

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  Staurosporine treated HeLa cells for 4 h.

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Product Information

A remarkable feature of apoptosis is the degradation of chromosome DNA, which is a common phenomenon. The degradation is very specific and regular. DNA fragments break into different lengths are of 180 bp-200 bp, showing a specific ladder like ladder pattern in agarose gel electrophoresis. The kit uses TUNEL method and uses Terminal Deoxynucleotidyl Transferase(TdT) catalyzes the incorporation of CoraLite®594-dUTP into the 3´-OH terminal of the broken DNA of apoptotic cells. DNA labeled with CoraLite®594- dUTP can be observed directly by fluorescence microscope or quantified by flow cytometry. TUNEL can selectively detect apoptotic cells rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy.

Components	20T	50T
CL594 TUNEL Reaction Buffer	2 × 0.5 mL	2 × 1.25 mL
TdT Enzyme	20 uL	50 uL
Proteinase K (2 mg/mL)	40 uL	100 uL
DNase I (2 U/μL）	5 uL	13 uL
10 X DNase I Buffer	100 uL	260 uL

Storage

Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.

Protocol

CoraLite 594 TUNEL Apoptosis Protocol

Cautions

TUNEL Equilibration Buffer and TUNEL Reaction Buffer contain toxic and carcinogenic ingredients. Please wear masks and gloves when using them. After contact with the skin, please rinse immediately with plenty of water. Dispose of waste liquid as toxic substances. Please try to avoid light during storage. For your safety and health, please wear lab coats and disposable gloves for operation.

Conjugated

CoraLite®594 mark, CL594 for short, this dye is similar to FITC and other dyes.

Ex/Em: 593/614 nm

Cited in Article as

PF00009, CoraLite®594 TUNEL Assay Apoptosis Detection Kit, Proteintech, IL, USA

Publications

Application	Title
	Oxid Med Cell Longev; TSPO Ligands Protect against Neuronal Damage Mediated by LPS-Induced BV-2 Microglia Activation.; Authors - Baoyu Ma
	Front Cell Neurosci; Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.; Authors - Lin-Yan Huang
	J Adv Res; Metrnl ameliorates diabetic cardiomyopathy via inactivation of cGAS/STING signaling dependent on LKB1/AMPK/ULK1-mediated autophagy; Authors - Qing-Bo Lu
	CNS Neurosci Ther; The cold-inducible RNA-binding protein-Thioredoxin 1 pathway ameliorates mitochondrial dysfunction and mitochondrial dynamin-related protein 1 level in the hippocampus of aged mice with perioperative neurocognitive dysfunction; Authors - Jingyao Huang