CoraLite®594 TUNEL Assay Apoptosis Detection Kit

Excitation Laser

593 nm

Emission

614 nm

Application

FC

Cat no : PF00009



Product Information

A remarkable feature of apoptosis is the degradation of chromosome DNA, which is a common phenomenon. The degradation is very specific and regular. DNA fragments break into different lengths are of 180 bp-200 bp, showing a specific ladder like ladder pattern in agarose gel electrophoresis. The kit uses TUNEL method and uses Terminal Deoxynucleotidyl Transferase(TdT) catalyzes the incorporation of CoraLite®594-dUTP into the 3´-OH terminal of the broken DNA of apoptotic cells. DNA labeled with CoraLite®594- dUTP can be observed directly by fluorescence microscope or quantified by flow cytometry. TUNEL can selectively detect apoptotic cells rather than necrotic cells or cells with DNA strand breaks caused by irradiation and drug therapy.

   Components

20T

50T

 TUNEL Equilibration Buffer

2 X 1 mL

5 mL

 CL594 TUNEL Reaction Buffer

2 X 0.5 mL

5 X 0.5 mL

 TdT Enzyme

20 μL

50 μL

 Proteinase K (2 mg/mL)

40 μL

100 μL

 DNase I (2 U/μL)

5 μL

13 μL

 10 X DNase I Buffer

100 μL

260 μL


Storage

Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Avoid freeze- thaw cycles.

Cautions

TUNEL Equilibration Buffer and TUNEL Reaction Buffer contain toxic and carcinogenic ingredients. Please wear masks and gloves when using them. After contact with the skin, please rinse immediately with plenty of water. Dispose of waste liquid as toxic substances. Please try to avoid light during storage. For your safety and health, please wear lab coats and disposable gloves for operation.

Conjugated

CoraLite®594 mark, CL594 for short, this dye is similar to FITC and other dyes.

Ex/Em: 593/614 nm