Cyclin D1 Polyclonal antibody
Cyclin D1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
186
Cat no : 26939-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, C6 cells, MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human oesophagus cancer tissue, mouse pancreas tissue, human ovary tumor tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 183 publications below |
IHC | See 9 publications below |
Product Information
The immunogen of 26939-1-AP is Cyclin D1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine, pig, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cyclin D1 fusion protein Ag25393 |
Full Name | cyclin D1 |
Calculated molecular weight | 295 aa, 34 kDa |
Observed molecular weight | 34 kDa |
GenBank accession number | BC000076 |
Gene symbol | CCND1 |
Gene ID (NCBI) | 595 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cyclin D1 antibody 26939-1-AP | Download protocol |
IHC protocol for Cyclin D1 antibody 26939-1-AP | Download protocol |
IF protocol for Cyclin D1 antibody 26939-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EBioMedicine PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness. | ||
Biomed Res Int UHRF1 Promotes Proliferation of Human Adipose-Derived Stem Cells and Suppresses Adipogenesis via Inhibiting Peroxisome Proliferator-Activated Receptor γ. | ||
J Exp Clin Cancer Res Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop. | ||
Cancer Manag Res Oxymatrine Inhibits the Proliferation and Invasion of Breast Cancer Cells via the PI3K Pathway. | ||
Oncotarget GOLPH3 is a potential therapeutic target and a prognostic indicator of poor survival in bladder cancer treated by cystectomy. |