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- KD/KO Validated
Cyclin D1 Polyclonal antibody
Cyclin D1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 26939-1-AP
Validation Data Gallery
|Positive WB detected in||LNCaP cells, MCF-7 cells, C6 cells, NIH/3T3 cells, HeLa cells, Neuro-2a cells, mouse lung tissue|
|Positive IHC detected in||human ovary tumor tissue, human oesophagus cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||SH-SY5Y cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:750-1:3000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
26939-1-AP targets Cyclin D1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, sheep, pig, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin D1 fusion protein Ag25393|
|Full Name||cyclin D1|
|Calculated molecular weight||295 aa, 34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC000076|
|Gene ID (NCBI)||595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
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