Cyclin D1 Polyclonal antibody

Cyclin D1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

186

Cat no : 26939-1-AP

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, Cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

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Validation Data Gallery

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  WB analysis using 26939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26939-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 26939-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 26939-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 26939-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: LNCaP cells, C6 cells, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human oesophagus cancer tissue, mouse pancreas tissue, human ovary tumor tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 183 publications below
• IHC: See 9 publications below

Product Information

The immunogen of 26939-1-AP is Cyclin D1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin D1 fusion protein Ag25393
• Full Name: cyclin D1
• Calculated molecular weight: 295 aa, 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC000076
• Gene symbol: CCND1
• Gene ID (NCBI): 595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Publications

Species	Application	Title
human	WB	EBioMedicine; PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.; Authors - Daojiang Li
human	WB	Biomed Res Int; UHRF1 Promotes Proliferation of Human Adipose-Derived Stem Cells and Suppresses Adipogenesis via Inhibiting Peroxisome Proliferator-Activated Receptor γ.; Authors - Ke Chen
human	WB	J Exp Clin Cancer Res; Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop.; Authors - Yan Liu
human	WB	Med Sci Monit; PMID: 31761897; Authors - Yangle Li
human	WB	Cancer Manag Res; Oxymatrine Inhibits the Proliferation and Invasion of Breast Cancer Cells via the PI3K Pathway.; Authors - Lin Guo
human	WB	Oncotarget; GOLPH3 is a potential therapeutic target and a prognostic indicator of poor survival in bladder cancer treated by cystectomy.; Authors - Qing Zhang

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