Cyclin D1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cyclin D1 Polyclonal Antibody for FC (Intra), IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

251

Cat no : 26939-1-AP

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, Cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

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Validation Data Gallery

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  WB analysis using 26939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26939-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 26939-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 26939-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 26939-1-AP (Cyclin D1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 26939-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 26939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Cyclin D1 antibody (26939-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 26939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26939-1-AP (Cyclin D1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 26939-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cyclin D1 (26939-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells, C6 cells
• Positive IHC detected in: human ovary tumor tissue, human oesophagus cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 248 publications below
• IHC: See 14 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26939-1-AP is Cyclin D1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cyclin D1 fusion protein Ag25393
• Full Name: cyclin D1
• Calculated molecular weight: 295 aa, 34 kDa
• Observed molecular weight: 34 kDa
• GenBank accession number: BC000076
• Gene symbol: CCND1
• Gene ID (NCBI): 595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Publications

Species	Application	Title
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Nat Commun; Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.; Authors - Dan Su
human	WB	J Crohns Colitis; M2 macrophage-derived exosomal-miR-590-3p attenuates DSS-induced mucosal damage and promotes epithelial repair via the LATS1/YAP/β-catenin signalling axis.; Authors - Feihong Deng
human	WB	Oncogene; Disrupted mitochondrial homeostasis coupled with mitotic arrest generates antineoplastic oxidative stress; Authors - Xiaohe Hao
human	WB	Clin Transl Med; FL118, acting as a 'molecular glue degrader', binds to dephosphorylates and degrades the oncoprotein DDX5 (p68) to control c-Myc, survivin and mutant Kras against colorectal and pancreatic cancer with high efficacy.; Authors - Xiang Ling

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