Cyclin D1 Polyclonal antibody

Cyclin D1 Polyclonal Antibody for FC, IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26939-1-AP

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, Cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31



Tested Applications

Positive WB detected inMCF-7 cells, LNCaP cells, C6 cells
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26939-1-AP is Cyclin D1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin D1 fusion protein Ag25393
Full Name cyclin D1
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000076
Gene symbol CCND1
Gene ID (NCBI) 595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.

Protocols

Product Specific Protocols
WB protocol for Cyclin D1 antibody 26939-1-APDownload protocol
IF protocol for Cyclin D1 antibody 26939-1-APDownload protocol
FC protocol for Cyclin D1 antibody 26939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

PLAGL2 and POFUT1 are regulated by an evolutionarily conserved bidirectional promoter and are collaboratively involved in colorectal cancer by maintaining stemness.

Authors - Daojiang Li
humanWB

Biomed Res Int

UHRF1 Promotes Proliferation of Human Adipose-Derived Stem Cells and Suppresses Adipogenesis via Inhibiting Peroxisome Proliferator-Activated Receptor γ.

Authors - Ke Chen
humanWB

J Exp Clin Cancer Res

Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop.

Authors - Yan Liu
humanWB

Med Sci Monit

PMID: 31761897

Authors - Yangle Li
humanWB

Cancer Manag Res

Oxymatrine Inhibits the Proliferation and Invasion of Breast Cancer Cells via the PI3K Pathway.

Authors - Lin Guo
humanWB

Oncotarget

GOLPH3 is a potential therapeutic target and a prognostic indicator of poor survival in bladder cancer treated by cystectomy.

Authors - Qing Zhang