Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, RAW 264.7 cells, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10109-2-AP targets DAB2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0162 Product name: Recombinant human DAB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 340-580 aa of BC003064 Sequence: VDYFGQQFDQISNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSVQSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTSAPAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSPFAASTPPPVPVVWGPSASVA Predict reactive species |
| Full Name | disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 96 kDa |
| GenBank Accession Number | BC003064 |
| Gene Symbol | DAB2 |
| Gene ID (NCBI) | 1601 |
| RRID | AB_2089700 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98082 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DAB2 antibody 10109-2-AP | Download protocol |
| IF protocol for DAB2 antibody 10109-2-AP | Download protocol |
| IHC protocol for DAB2 antibody 10109-2-AP | Download protocol |
| IP protocol for DAB2 antibody 10109-2-AP | Download protocol |
| WB protocol for DAB2 antibody 10109-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DAB2 antibody (Cat# 10109-2-AP) has 11 citations published in journals including Theranostics, Science Advances, Cancer Letters, PNAS, Immunotargets and Therapy, Journal of Biological Chemistry, Journal of Cellular Physiology, Histopathology, Journal of Diabetes Research, Disease Markers, and Folia Histochemica et Cytobiologica. Research fields span liver cancer, KSHV infection, nasopharyngeal carcinoma, intraflagellar transport, gastric cancer, transcriptional regulation, colon pathology, endometrial cancer, renal tubular reabsorption, thyroid carcinoma, and adrenal gland biology.
| Species | Application | Title |
|---|---|---|
Theranostics DAB2 + macrophages support FAP + fibroblasts in shaping tumor barrier and inducing poor clinical outcomes in liver cancer | ||
Sci Adv Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis
| ||
Cancer Lett MicroRNA-93 promotes cell growth and invasion in nasopharyngeal carcinoma by targeting disabled homolog-2. | ||
Proc Natl Acad Sci U S A FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia. | ||
J Biol Chem Nuclear myosin VI cooperates with actin to promote transcriptional cluster formation at androgen receptors. | ||
Immunotargets Ther Comprehensive Analyses of Single-Cell and Bulk RNA Sequencing Data From M2 Macrophages to Elucidate the Immune Prognostic Signature in Patients with Gastric Cancer Peritoneal Metastasis |
Reviews
What customers say
The single review for this product is positive, with a 5-star rating. The customer used it for Western Blot at a 1:1000 dilution on mouse tissue and noted that the result was a little bit dirty at that dilution but still acceptable. Overall, the feedback suggests the antibody performs adequately for WB, though users may want to optimize dilution to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bruna (Verified Customer) (06-18-2026) | A litlle bit dirty at 1:1000 but ok
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