DAPP1 Recombinant antibody, PBS Only

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  WB analysis using 85346-1-RR (same clone as 85346-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85346-1-RR (DAPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. Jurkat cells as negative control. This data was developed using the same antibody clone with 85346-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85346-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85346-1-RR against Human DAPP1 were performed. The affinity constant is 8.12 nM.

DAPP1 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85346-1-PBS

Clone No.242582D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

BAM32, B-cell adapter molecule of 32 kDa, DAPP 1, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, hDAPP1

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Product Information

85346-1-PBS targets DAPP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DAPP1 fusion protein Ag6459
• Full Name: dual adaptor of phosphotyrosine and 3-phosphoinositides
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC012924
• Gene Symbol: DAPP1
• Gene ID (NCBI): 27071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UN19
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DAPP1 was identified as dual adaptor for phosphotyrosine and 3-phosphoinositides, a novel 280 amino acid protein which contains a putative myristoylation site at its N-terminus followed by a SH2 and a PH domain at its C-terminus (PMID: 10432293). DAPP1 is also referred to B cell adaptor molecule of 32 kDa (Bam32), and is expressed by B lymphocytes, but not T lymphocytes or nonhematopoietic cells. DAPP1/Bam32 was shown to regulate BCR signaling downstream of phosphatidylinositol 3-kinase (PI3K) (PMID: 10770799). Furthermore, DAPP1/Bam32 may server to integrate PI3K and Src kinase signaling to promote Rac-dependent B cell adhesive interactions important for antigen presentation function (PMID: 20495066).