|Positive WB detected in||human brain tissue, HepG2 cells, HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16019-1-AP targets DBR1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||DBR1 fusion protein Ag8830|
|Full Name||debranching enzyme homolog 1 (S. cerevisiae)|
|Calculated molecular weight||544 aa, 62 kDa|
|Observed molecular weight||70-80 kDa|
|GenBank accession number||BC009472|
|Gene ID (NCBI)||51163|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.
Int J Mol Sci
Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.
Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus.
Mol Cell Proteomics
Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.
Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.
Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marta (Verified Customer) (02-18-2020)
Single band in the WB at the expected size, incubated overnight at 4C with a 1/1200 dilution
Aiswarya (Verified Customer) (12-23-2019)
The Dbr1 antibody was used to check knock down of Dbr1 and worked well. Additional non specific bands come up but do not interfere in the specific size range.