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  • KD/KO Validated

DBR1 Polyclonal antibody

DBR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16019-1-AP

Synonyms

DBR1, Lariat debranching enzyme



Tested Applications

Positive WB detected inhuman brain tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16019-1-AP targets DBR1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBR1 fusion protein Ag8830
Full Name debranching enzyme homolog 1 (S. cerevisiae)
Calculated molecular weight 544 aa, 62 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009472
Gene symbol DBR1
Gene ID (NCBI) 51163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DBR1 antibody 16019-1-APDownload protocol
IHC protocol for DBR1 antibody 16019-1-APDownload protocol
IF protocol for DBR1 antibody 16019-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Int J Mol Sci

Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

Authors - So Masaki
humanIF

PLoS One

Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus.

Authors - Anne Hett
humanWB

Mol Cell Proteomics

Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.

Authors - Syred Heather M HM
humanWB

Cell

Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.

Authors - Shen-Ying Zhang
humanWB

Nat Genet

Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.

Authors - Armakola Maria M
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marta (Verified Customer) (02-18-2020)

Single band in the WB at the expected size, incubated overnight at 4C with a 1/1200 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1200
  • Cell Tissue Type: PBMC, B-EBV cells
DBR1 Antibody Western Blot, validation (1/1200 dilution) in PBMC, B-EBV cells (Cat no:16019-1-AP)
FH

Aiswarya (Verified Customer) (12-23-2019)

The Dbr1 antibody was used to check knock down of Dbr1 and worked well. Additional non specific bands come up but do not interfere in the specific size range.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HAP1, HEK293