DBR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DBR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (2)

Cat no : 16019-1-AP

Synonyms

Lariat debranching enzyme, EC:3.1.4.-

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Validation Data Gallery

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  WB analysis of HeLa using 16019-1-AP

  WB result of DBR1 antibody (16019-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DBR1 transfected HeLa cells.

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  WB analysis of human brain using 16019-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16019-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16019-1-AP (DBR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16019-1-AP

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:800) with HeLa cells lysate 1560 ug.

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  IP experiment of mouse brain using 16019-1-AP

  IP result of anti-DBR1 (IP:16019-1-AP, 4ug; Detection:16019-1-AP 1:500) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16019-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16019-1-AP (DBR1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16019-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DBR1 antibody (16019-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, HeLa cells
• Positive IP detected in: mouse brain tissue, HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

16019-1-AP targets DBR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DBR1 fusion protein Ag8830
• Full Name: debranching enzyme homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 544 aa, 62 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC009472
• Gene Symbol: DBR1
• Gene ID (NCBI): 51163
• RRID: AB_2230316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DBR1(Lariat debranching enzyme) hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules. DBR1 belongs to the lariat debranching enzyme family. Inhibiton of Dbr1 can suppress TDP-43 toxicity in primary neurons, suggesting that Dbr1 could be a potential therapeutic target for ALS and related TDP-43 proteinopathies (PMID: 23104007). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Genet; Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.; Authors - Armakola Maria M ; KD Validated
human	WB	Cell; Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.; Authors - Shen-Ying Zhang
human	WB	J Exp Med; SARS-CoV-2 brainstem encephalitis in human inherited DBR1 deficiency; Authors - Yi-Hao Chan
human	WB	Mol Cell Proteomics; Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.; Authors - Syred Heather M HM
human	IF	PLoS One; Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus.; Authors - Anne Hett
human	WB, IHC	Int J Mol Sci; Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.; Authors - So Masaki

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marta (Verified Customer) (02-18-2020)

Single band in the WB at the expected size, incubated overnight at 4C with a 1/1200 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1200
• Cell Tissue Type: PBMC, B-EBV cells

DBR1 Antibody Western Blot, validation (1/1200 dilution) in PBMC, B-EBV cells (Cat no:16019-1-AP)

FH

Aiswarya (Verified Customer) (12-23-2019)

The Dbr1 antibody was used to check knock down of Dbr1 and worked well. Additional non specific bands come up but do not interfere in the specific size range.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1, HEK293