DFFA/DFF45 Polyclonal antibody, PBS Only

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  WB analysis using 10191-2-AP (same clone as 10191-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

  IP result of anti-DFF45/DFFA (IP:10191-2-AP, 3ug; Detection:10191-2-AP 1:1000) with HEK-293 cells lysate 2500ug. This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10191-2-AP (same clone as 10191-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DFFA/DFF45 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10191-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

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Product Information

10191-2-PBS targets DFFA/DFF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DFFA/DFF45 fusion protein Ag0244
• Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa, 45 kDa
• GenBank Accession Number: BC000037
• Gene Symbol: DFFA
• Gene ID (NCBI): 1676
• RRID: AB_2292937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00273
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.