DLK1 Polyclonal antibody

• 

  WB analysis of mouse placenta using 10636-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10636-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10636-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 10636-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10636-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10636-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10636-1-AP (DLK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10636-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10636-1-AP (DLK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10636-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10636-1-AP (DLK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10636-1-AP (DLK1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10636-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10636-1-AP (DLK1 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

DLK1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10636-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Pref 1, pG2, FA1, DLK-1, DLK 1

---

Tested Applications

• Positive WB detected in: mouse placenta tissue, mouse ovary tissue, 3T3-L1 cells, MCF-7 cells, A549 cells, mouse brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 11 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

10636-1-AP targets DLK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLK1 fusion protein Ag0991
• Full Name: delta-like 1 homolog (Drosophila)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC007741
• Gene Symbol: DLK1
• Gene ID (NCBI): 8788
• RRID: AB_2092679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P80370
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).

Publications

Species	Application	Title
mouse	IHC	Gastroenterology; Activation of β-catenin and Yap1 in human hepatoblastoma and induction of hepatocarcinogenesis in mice.; Authors - Junyan Tao
human	IHC	Nat Commun; GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling.; Authors - Shinji Matsumoto
mouse	WB	PLoS Genet; Gene dosage effects of the imprinted delta-like homologue 1 (dlk1/pref1) in development: implications for the evolution of imprinting.; Authors - da Rocha Simao Teixeira ST
human	WB	Cell Death Differ; The miR-181d-regulated metalloproteinase Adamts1 enzymatically impairs adipogenesis via ECM remodeling.; Authors - S-Z Chen
pig	IHC	Meat Sci; Dietary vitamin A restriction affects adipocyte differentiation and fatty acid composition of intramuscular fat in Iberian pigs.; Authors - M Ayuso
mouse	IHC	Front Cell Dev Biol; A Na+/K+ ATPase Pump Regulates Chondrocyte Differentiation and Bone Length Variation in Mice.; Authors - Marta Marchini

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Angélique (Verified Customer) (12-04-2019)

Good specific signal without background staining in IHC on mouse liver and liver tumor sections.

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1/100 in IHC, 1/500 in WB
• Cell Tissue Type: mouse hepatocytes and liver tumours

DLK1 Antibody Western Blot,Immunohistochemistry, validation (1/100 in IHC, 1/500 in WB dilution) in mouse hepatocytes and liver tumours (Cat no:10636-1-AP)