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DLK1 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 10636-1-AP

Featured Product KD/KO validated

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

DLK1 Antibody WB mouse placenta tissue 10636-1-AP

mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10636-1-AP(DLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

DLK1 Antibody IHC human breast cancer tissue 10636-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10636-1-AP(DLK1 antibody) at dilution of 1:50 (under 10x lens)

DLK1 Antibody IHC human breast cancer tissue 10636-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10636-1-AP(DLK1 antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
mouse placenta tissue, A549 cells, MCF-7 cells, mouse brain tissue, mouse ovary tissue
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DLK1 fusion protein Ag0991
Full name:
delta-like 1 homolog (Drosophila)
Calculated molecular weight:
41 kDa
Observed molecular weight:
45-60 kDa
GenBank accession number:
BC007741
Gene ID (NCBI):
8788
Gene symbol
DLK1
Synonyms
DLK, DLK 1, DLK1, FA1, pG2, Pref 1, PREF1, Protein delta homolog 1, ZOG

Background

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).


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