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  • KD/KO Validated

DLK1 Antibody

DLK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 10636-1-AP

Synonyms

DLK, DLK 1, DLK1, FA1, pG2, Pref 1, PREF1, Protein delta homolog 1, ZOG



Tested Applications

Positive WB detected inmouse placenta tissue, mouse ovary tissue, 3T3-L1 cells, MCF-7 cells, A549 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

10636-1-AP targets DLK1 in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLK1 fusion protein Ag0991
Full Name delta-like 1 homolog (Drosophila)
Calculated molecular weight 41 kDa
Observed molecular weight 45-60 kDa
GenBank accession number BC007741
Gene symbol DLK1
Gene ID (NCBI) 8788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).

Protocols

Product Specific Protocols
WB protocol for DLK1 antibody 10636-1-APDownload protocol
IHC protocol for DLK1 antibody 10636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Pathol

TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma.

Authors - Jie Zhang
mouseWB

Nephrology (Carlton)

Visceral adipogenesis inhibited by Pref-1 is associated with peritoneal angiogenesis.

Authors - Ran Jing
pigIHC

Meat Sci

Dietary vitamin A restriction affects adipocyte differentiation and fatty acid composition of intramuscular fat in Iberian pigs.

Authors - M Ayuso
humanWB,IHC

Biosci Rep

Abnormal localized DLK1 interacts with NCOR1 in non-small cell lung cancer cell nuclear.

Authors - Jinjing Tan
ratIHC

J Neuroendocrinol

Delta-like 1 homologue is a hypothalamus-enriched protein that is present in orexin-containing neurones of the lateral hypothalamic area.

Authors - Meister B B
humanWB

PLoS One

DLK1 Promotes Lung Cancer Cell Invasion through Upregulation of MMP9 Expression Depending on Notch Signaling.

Authors - Lin Li
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Angélique (Verified Customer) (12-04-2019)

Good specific signal without background staining in IHC on mouse liver and liver tumor sections.

  • Applications: Western Blot,Immunohistochemistry,
  • Primary Antibody Dilution: 1/100 in IHC, 1/500 in WB
  • Cell Tissue Type: mouse hepatocytes and liver tumours
DLK1 Antibody Western Blot,Immunohistochemistry, validation (1/100 in IHC, 1/500 in WB dilution) in mouse hepatocytes and liver tumours (Cat no:10636-1-AP)