|Positive WB detected in||mouse liver tissue, HEK-293 cells, human liver tissue, mouse spleen tissue, HeLa cells, COLO 320 cells|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:600-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
13174-1-AP targets DNAJB1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAJB1 fusion protein Ag3830|
|Full Name||DnaJ (Hsp40) homolog, subfamily B, member 1|
|Calculated molecular weight||340 aa, 40 kDa|
|Observed molecular weight||37-40 kDa|
|GenBank accession number||BC019827|
|Gene ID (NCBI)||3337|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.
|Product Specific Protocols|
|WB protocol for DNAJB1 antibody 13174-1-AP||Download protocol|
|IHC protocol for DNAJB1 antibody 13174-1-AP||Download protocol|
|IF protocol for DNAJB1 antibody 13174-1-AP||Download protocol|
|IP protocol for DNAJB1 antibody 13174-1-AP||Download protocol|
|FC protocol for DNAJB1 antibody 13174-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ana (Verified Customer) (03-11-2021)
DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.