• Featured Product
  • KD/KO Validated

DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 13174-1-AP

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, human liver tissue, mouse spleen tissue, HeLa cells, COLO 320 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13174-1-AP targets DNAJB1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated molecular weight 340 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC019827
Gene symbol DNAJB1
Gene ID (NCBI) 3337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
WB protocol for DNAJB1 antibody 13174-1-APDownload protocol
IHC protocol for DNAJB1 antibody 13174-1-APDownload protocol
IF protocol for DNAJB1 antibody 13174-1-APDownload protocol
IP protocol for DNAJB1 antibody 13174-1-APDownload protocol
FC protocol for DNAJB1 antibody 13174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Front Aging Neurosci

Crosstalk Between Chaperone-Mediated Protein Disaggregation and Proteolytic Pathways in Aging and Disease.

Authors - Diogo R Feleciano
  • KD Validated
mouseWB

Front Genet

Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain.

Authors - Wenyue Su
mouseWB

Geroscience

Augmentation of the heat shock axis during exceptional longevity in Ames dwarf mice.

Authors - Rachana Trivedi
humanWB

Int J Mol Sci

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Authors - Yanqing Li
humanWB

J Toxicol Sci

Reactive metabolite of gefitinib activates inflammasomes: implications for gefitinib-induced idiosyncratic reaction.

Authors - Ryuji Kato
mouseWB

Mol Reprod Dev

Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.

Authors - Lihua Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana (Verified Customer) (03-11-2021)

DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver & Heart
DNAJB1 Antibody Western Blot validation (1:1000 dilution) in Mouse Liver & Heart (Cat no:13174-1-AP)