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  • KD/KO Validated

DNAJB1 Polyclonal antibody

DNAJB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 13174-1-AP

Synonyms

DnaJ protein homolog 1, DNAJ1, DNAJB1, hDj 1, Hdj1, Heat shock 40 kDa protein 1, Heat shock protein 40, Hsp40, HSPF1, Human DnaJ protein 1, Sis1



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse lung tissue, rat lung tissue, rat liver tissue, C2C12 cells, C6 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman oesophagus cancer tissue, human lymphoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13174-1-AP targets DNAJB1 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJB1 fusion protein Ag3830
Full Name DnaJ (Hsp40) homolog, subfamily B, member 1
Calculated Molecular Weight 340 aa, 40 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC019827
Gene Symbol DNAJB1
Gene ID (NCBI) 3337
RRIDAB_2230742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

Protocols

Product Specific Protocols
WB protocol for DNAJB1 antibody 13174-1-APDownload protocol
IHC protocol for DNAJB1 antibody 13174-1-APDownload protocol
IF protocol for DNAJB1 antibody 13174-1-APDownload protocol
IP protocol for DNAJB1 antibody 13174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
mouseWB

Mol Ther Nucleic Acids

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction

Authors - Jie Pan
humanWB

Int J Mol Sci

PGC1α Promotes Cisplatin Resistance in Ovarian Cancer by Regulating the HSP70/HK2/VDAC1 Signaling Pathway.

Authors - Yanqing Li
mouseWB

Front Mol Neurosci

Inhibition of Heat Shock Protein 90 by 17-AAG Reduces Inflammation via P2X7 Receptor/NLRP3 Inflammasome Pathway and Increases Neurogenesis After Subarachnoid Hemorrhage in Mice.

Authors - Yuchun Zuo
mouseWB

J Gerontol A Biol Sci Med Sci

Multifactorial Attenuation of the Murine Heat Shock Response With Age.

Authors - Donald A Jurivich
mouseWB

Biogerontology

Disrupted HSF1 regulation in normal and exceptional brain aging

Authors - Rachana Trivedi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana (Verified Customer) (03-11-2021)

DNJB1 in mouse samples. 10 ug of total protein. 1:1000 O/N 4ºC incubation in BSA 3% PBST. Secondary ab HRP conjugated 1:5000 1h RT. 2 bands observed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Liver & Heart
DNAJB1 Antibody Western Blot validation (1:1000 dilution) in Mouse Liver & Heart (Cat no:13174-1-AP)