DNMT1 Monoclonal antibody

DNMT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat and More (1)







Cat no : 68485-1-Ig


AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68485-1-Ig targets DNMT1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNMT1 fusion protein Ag19116
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated Molecular Weight 1632 aa, 185 kDa
Observed Molecular Weight180-200 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with MW 184 kDa and 145 kDa. (PMID: 10753866)


Product Specific Protocols
WB protocol for DNMT1 antibody 68485-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database

Authors - Jianjun Wu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hua (Verified Customer) (04-26-2024)

Works very well in WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver and mouse brain
DNMT1 Antibody Western Blot validation (1:1000 dilution) in mouse liver and mouse brain (Cat no:68485-1-Ig)