Rat E-Cadherin Recombinant antibody, PBS Only

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  WB analysis using 84646-4-RR (same clone as 84646-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84646-4-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84646-4-RR (same clone as 84646-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84646-4-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 84646-4-RR (same clone as 84646-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 84646-4-RR (same clone as 84646-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84646-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84646-4-RR against Rat E-Cadherin were performed. The affinity constant is 1.38 nM.

E-Cadherin {ptg:Brand} Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84646-4-PBS

Clone No.242018C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

242018C10

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Product Information

84646-4-PBS targets E-Cadherin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cadherin 1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_031334.1
• Gene Symbol: Cdh1
• Gene ID (NCBI): 83502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9R0T4
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.