Filter: AllWBIHCIF-PAffinity

WB analysis using 84646-4-RR (same clone as 84646-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84646-4-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
WB analysis using 84646-4-RR (same clone as 84646-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84646-4-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IF Staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using E-Cadherin antibody (84646-4-RR, Clone: 242018C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using E-Cadherin antibody (84646-4-RR, Clone: 242018C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
IF Staining of mouse colon using 84646-4-RR (same clone as 84646-4-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-Cadherin antibody (84646-4-RR, Clone: 242018C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-Cadherin antibody (84646-4-RR, Clone: 242018C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 84646-4-RR
Biolayer interferometry (BLl) kinetic assays of 84646-4-RR against Rat E-Cadherin were performed. The affinity constant is 1.38 nM.

Rat E-cadherin Recombinant monoclonal antibody, PBS Only

E-cadherin Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84646-4-PBS

Clone No.242018C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242018C10, Cadherin-1, CD324, Cdh1, E-Cad/CTF1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84646-4-PBS targets E-cadherin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1229 Product name: Recombinant Rat E-Cadherin protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 24-713 aa of NM_031334.1 Sequence: QPESESDSCRPGFSSEVYTFLVPERHLERGHILGRVKFEGCTGRPRTAFFSEDSRFKVSTDGVITVKRHLKLHKLETSFLVHAWDSSYRKLSTKVTLKSLGHHHHRHHHRDPVSESNPELLTFPSFHQGLRRQKRDWVIPPINCPENQKGEFPQRLVQIKSNRDKETTVFYSITGPGADKPPVGVFIIERETGWLKVTQPLDREAIDKYLLYSHAVSSNGEAVEDPMEIVVTVTDQNDNRPEFIQEVFEGSVAEGALPGTSVMQVSATDADDDINTYNAAIAYTILSQDPELPHKNMFTVNRDTGVISVVTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPIFNPSTYQGQVLENEVGARIATLKVTDDDAPNTPAWNAVYTVVNDPDHQFTVITDPKTNEGILKTAKGLDFEAKQQYILHVTVENEEPFEGSLVPSTATVTVDVVDVNEAPIFVPAEKRVEVPEDFGVGLEIASYTAREPDTFMEQKITYRIWRDTANWLEINPETGVISTRAEMDREDSEHVKNSTYTALIIATDDGSPIATGTGTLLLVLSDVNDNAPIPEPRNMQFCQRNPKPHVITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAEQESLILQPRKDLEIGEYKINLKLSDNQNKDQVTTLEVHVCDCEGTVNNCMKAISLEAGLQVPA Predict reactive species
Full Name	cadherin 1
Calculated Molecular Weight	99 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	NM_031334.1
Gene Symbol	Cdh1
Gene ID (NCBI)	83502
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q9R0T4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.