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E-cadherin Polyclonal antibody
E-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20874-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, DU 145 cells, mouse testis tissue, MCF-7 cells, T-47D cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human breast cancer tissue, human colon tissue, mouse colon tissue, mouse skin tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells, mouse small intestine tissue, human breast cancer tissue, mouse embryo tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20874-1-AP is E-cadherin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, canine, pig, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||E-cadherin fusion protein Ag14973|
|Full Name||cadherin 1, type 1, E-cadherin (epithelial)|
|Calculated molecular weight||882 aa, 97 kDa|
|Observed molecular weight||120-125 kDa|
|GenBank accession number||BC141838|
|Gene ID (NCBI)||999|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.
|Product Specific Protocols|
|WB protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IHC protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IF protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|IP protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|FC protocol for E-cadherin antibody 20874-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Davide (Verified Customer) (11-22-2022)
The antibody works optimally at 1:100. By the strenght of the signal it could probably also work at 1:50. Optimal product. E-CAD in red in the picture (abundant background signal due to non optimal blocking)
Sophie (Verified Customer) (02-07-2022)
Highly efficient staining when used at 1/1000 overnight for IF on paraffin embedded tissues
Kishor (Verified Customer) (01-30-2019)
This antibody working very good for human cancer cells in western blotting (1:1000)
Martin (Verified Customer) (12-13-2017)