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RCAS1 Polyclonal antibody

RCAS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12255-1-AP

Synonyms

EB9, EBAG9, PDAF, RCAS1



Tested Applications

Positive WB detected inmouse heart tissue, HEK-293
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12255-1-AP targets RCAS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCAS1 fusion protein Ag2905
Full Name estrogen receptor binding site associated, antigen, 9
Calculated molecular weight 213 aa, 24 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC017729
Gene symbol EBAG9
Gene ID (NCBI) 9166
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

Protocols

Product Specific Protocols
WB protocol for RCAS1 antibody 12255-1-APDownload protocol
IHC protocol for RCAS1 antibody 12255-1-APDownload protocol
IF protocol for RCAS1 antibody 12255-1-APDownload protocol
IP protocol for RCAS1 antibody 12255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols