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  • KD/KO Validated

EEF1A1 Polyclonal antibody

EEF1A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11402-1-AP

Synonyms

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, GRAF 1EF, HNGC:16303, LENG7, PTI1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, mouse ovary tissue, mouse heart tissue, rat brain tissue, human brain tissue, Jurkat cells, SH-SY5Y cells, Neuro-2a cells, C2C12 cells, HepG2 cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11402-1-AP targets EEF1A1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014224
Gene symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Protocols

Product Specific Protocols
WB protocol for EEF1A1 antibody 11402-1-APDownload protocol
IHC protocol for EEF1A1 antibody 11402-1-APDownload protocol
IF protocol for EEF1A1 antibody 11402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Authors - Daniel Arango
mouseWB

Sci Adv

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Authors - Sefan Asamitsu
humanWB,IF

Cancer Res

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Authors - Xiuxia Liu
  • KD Validated
humanWB

Cell Chem Biol

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Authors - Yingjie Hou
humanWB,IP

Cancer Lett

N6-methyladenosine modification regulates imatinib resistance of gastrointestinal stromal tumor by enhancing the expression of multidrug transporter MRP1.

Authors - Kangjing Xu
humanWB

RNA Biol

Opposing roles of C/EBPα and eEF1A1 in Sp1-regulated miR-122 transcription.

Authors - Chunxian Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

  • Applications: Western Blot
  • Cell Tissue Type: Heart