EEF1A1 Polyclonal antibody

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  WB analysis of HeLa using 11402-1-AP

  WB result of EEF1A1 antibody (11402-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HeLa cells.

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  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11402-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11402-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 11402-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11402-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11402-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11402-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11402-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11402-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11402-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11402-1-AP (EEF1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11402-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11402-1-AP (EEF1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11402-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF1A1 antibody (11402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 11402-1-AP

  Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

EEF1A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11402-1-AP

Publications(29)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

eEF1A 1, EEF1A, EEF 1, EC:3.6.5.-, CCS3

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, mouse ovary tissue, mouse heart tissue, rat brain tissue, human brain tissue, Jurkat cells, SH-SY5Y cells, Neuro-2a cells, C2C12 cells, HepG2 cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

11402-1-AP targets EEF1A1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1A1 fusion protein Ag1938
• Full Name: eukaryotic translation elongation factor 1 alpha 1
• Calculated Molecular Weight: 462 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014224
• Gene Symbol: EEF1A1
• Gene ID (NCBI): 1915
• RRID: AB_2096966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P68104
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Publications

Species	Application	Title
human	WB	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Nat Commun; Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction; Authors - Minji Kim ; KD Validated
human,mouse	WB,IF	Nat Chem Biol; Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis; Authors - Yuyin Pan
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

• Applications: Western Blot
• Cell Tissue Type: Heart