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EEF1A1 Polyclonal antibody
EEF1A1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, RIP, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11402-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, mouse liver tissue, mouse skeletal muscle tissue, MCF-7 cells, mouse pancreas tissue, mouse ovary tissue, mouse heart tissue, rat brain tissue, human brain tissue, Jurkat cells, SH-SY5Y cells, Neuro-2a cells, C2C12 cells, HepG2 cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 14 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
RIP | See 1 publications below |
Product Information
11402-1-AP targets EEF1A1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EEF1A1 fusion protein Ag1938 |
Full Name | eukaryotic translation elongation factor 1 alpha 1 |
Calculated molecular weight | 462 aa, 50 kDa |
Observed molecular weight | 50 kDa |
GenBank accession number | BC014224 |
Gene symbol | EEF1A1 |
Gene ID (NCBI) | 1915 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.
Protocols
Product Specific Protocols | |
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WB protocol for EEF1A1 antibody 11402-1-AP | Download protocol |
IHC protocol for EEF1A1 antibody 11402-1-AP | Download protocol |
IF protocol for EEF1A1 antibody 11402-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome. | ||
Cancer Res The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.
| ||
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
Cancer Lett N6-methyladenosine modification regulates imatinib resistance of gastrointestinal stromal tumor by enhancing the expression of multidrug transporter MRP1. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tom (Verified Customer) (08-25-2020) | This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.
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