• Featured Product
  • KD/KO Validated

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18986-1-AP

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, MCF-7 cells, multi-cells, HeLa/RPE1 cells, HeLa cells, NCI-H1299 cells, A549 cells, L02 cells, NCL-H1299 cells
Positive IP detected inMCF-7 cells
Positive IF detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 134 kDa
Observed molecular weight 145-165 kDa
GenBank accession numberNM_005228
Gene symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_10596476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Protocols

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IF protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IP protocol for EGFR-Specific antibody 18986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
  • KD Validated
humanWB

Cancer Commun (Lond)

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

Authors - Xiaoteng Cui
humanIF

ACS Appl Mater Interfaces

On-Chip Replication of Extremely Early-Stage Tumor Behavior.

Authors - Chengpan Li
pigWB

Biosens Bioelectron

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Authors - Yi-Jyun Chen
humanWB, IHC, ChIP

Cancer Res

Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.

Authors - Mahelet Mamo
WB, IF

Curr Biol

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Authors - Nazim Ali

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marina (Verified Customer) (02-13-2023)

A stronger band can be observed slighly above 170 kDa and a fainter band below 130 kDa. Ponceau red is used as total protein loading control. Primary antibody dilution 1:500, overnight incubation at 4ºC. Exposure time 3 minutes.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human triple negative breast cancer cells.
EGFR-Specific Antibody Western Blot validation (1:500 dilution) in Human triple negative breast cancer cells. (Cat no:18986-1-AP)
FH

Clarisse (Verified Customer) (12-12-2022)

This antibody works very well on tissue fixed overnight at 4 degrees C with 2% PFA. No antigen retrieval required.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human cerebral organoids
FH

Morgan (Verified Customer) (10-26-2021)

I had luck with this antibody as seen from the image attached. It took a little bit of time to develop though. Maybe using more than 1:500 would be better. Unfortunately I couldn't get the phosphorylated version (p-EGF) to work though. I used OV90 (ovarian cancer) cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: OV90 ovarian cancer cells
EGFR-Specific Antibody Western Blot validation (1:500 dilution) in OV90 ovarian cancer cells (Cat no:18986-1-AP)
FH

K (Verified Customer) (12-20-2020)

worked well for WB (1:1000)IF (1:200)and IHC (1:200)

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Cell Tissue Type: mouse liver tissues. rat liver and human liver cells
EGFR-Specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation ( dilution) in mouse liver tissues. rat liver and human liver cells (Cat no:18986-1-AP)
FH

XB (Verified Customer) (10-29-2020)

It is OK to use it to probe EGFR (about 180 kD), but there are some unspecific bands at the lower part of the gel.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC12 cell
EGFR-Specific Antibody Western Blot validation (1:1000 dilution) in PC12 cell (Cat no:18986-1-AP)