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EGFR-Specific Polyclonal antibody
EGFR-Specific Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (4)
WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Cat no : 18986-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, MCF-7 cells, NCI-H1299 cells, A431 cells, HeLa cells, L02 cells, HeLa/RPE1 cells, multi-cells, NCL-H1299 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||A431 cells|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:750-1:3000|
|Sample-dependent, check data in validation data gallery|
18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Full Name||epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)|
|Calculated molecular weight||134 kDa|
|Observed molecular weight||145-165 kDa|
|GenBank accession number||NM_005228|
|Gene ID (NCBI)||1956|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.
|Product Specific Protocols|
|WB protocol for EGFR-Specific antibody 18986-1-AP||Download protocol|
|IHC protocol for EGFR-Specific antibody 18986-1-AP||Download protocol|
|IF protocol for EGFR-Specific antibody 18986-1-AP||Download protocol|
|IP protocol for EGFR-Specific antibody 18986-1-AP||Download protocol|
|FC protocol for EGFR-Specific antibody 18986-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marina (Verified Customer) (02-13-2023)
A stronger band can be observed slighly above 170 kDa and a fainter band below 130 kDa. Ponceau red is used as total protein loading control. Primary antibody dilution 1:500, overnight incubation at 4ºC. Exposure time 3 minutes.
Clarisse (Verified Customer) (12-12-2022)
This antibody works very well on tissue fixed overnight at 4 degrees C with 2% PFA. No antigen retrieval required.
Morgan (Verified Customer) (10-26-2021)
I had luck with this antibody as seen from the image attached. It took a little bit of time to develop though. Maybe using more than 1:500 would be better. Unfortunately I couldn't get the phosphorylated version (p-EGF) to work though. I used OV90 (ovarian cancer) cells
K (Verified Customer) (12-20-2020)
worked well for WB (1:1000)IF (1:200)and IHC (1:200)
XB (Verified Customer) (10-29-2020)
It is OK to use it to probe EGFR (about 180 kD), but there are some unspecific bands at the lower part of the gel.