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EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18986-1-AP

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1



Tested Applications

Positive WB detected inL02 cells, multi-cells, HeLa/RPE1 cells, NCI-H1299 cells, HeLa cells, HepG2 cells, MCF-7 cells, A431 cells
Positive IP detected inMCF-7 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 134 kDa
Observed molecular weight 145-165 kDa
GenBank accession numberNM_005228
Gene symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERBB1, also mamed as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Protocols

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IHC protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IP protocol for EGFR-Specific antibody 18986-1-APDownload protocol
FC protocol for EGFR-Specific antibody 18986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.

Authors - Zhaoyu Qin
mouseWB

Ecotoxicol Environ Saf

PMID: 30974244

Authors - Qi Wang
humanWB

Int J Oncol

Adaptive EGF expression sensitizes pancreatic cancer cells to ionizing radiation through activation of the cyclin D1/P53/PARP pathway.

Authors - Xiaoxing Liu
humanIF

Development

The exocyst functions in niche cells to promote germline stem cell differentiation by directly controlling EGFR membrane trafficking.

Authors - Ying Mao
humanWB

Int J Cancer

VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer.

Authors - Yiyu Chen
humanWB

Front Oncol

CAPE-pNO2 Inhibited the Growth and Metastasis of Triple-Negative Breast Cancer via the EGFR/STAT3/Akt/E-Cadherin Signaling Pathway.

Authors - Qin Huang