Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human breast cancer tissue, mouse adrenal gland tissue, rat ovary tissue, mouse brain tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67674-1-Ig targets EIF2AK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16970 Product name: Recombinant human EIF2AK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 281-630 aa of BC006524 Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 1 |
| Calculated Molecular Weight | 630 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC006524 |
| Gene Symbol | EIF2AK1 |
| Gene ID (NCBI) | 27102 |
| RRID | AB_2882869 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BQI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2AK1 antibody 67674-1-Ig | Download protocol |
| IHC protocol for EIF2AK1 antibody 67674-1-Ig | Download protocol |
| WB protocol for EIF2AK1 antibody 67674-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF2AK1 Monoclonal antibody (2H1F3), catalog number 67674-1-Ig, has 1 total citation. It appears in Biochemical Pharmacology (Impact Factor 6.5), published in 2026. The cited study investigates how KCMF1 regulates HRI ubiquitination to inhibit the integrated stress response in ovarian cancer, using this antibody for Western blot and immunohistochemistry in human samples. The research falls within the fields of ovarian cancer biology and integrated stress response signaling.
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol KCMF1 regulates HRI ubiquitination to inhibit the integrated stress response in ovarian cancer. |
