EIF2AK1 Monoclonal antibody, PBS Only

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  WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67674-1-Ig (same clone as 67674-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

EIF2AK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67674-1-PBS

Clone No.2H1F3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

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Product Information

67674-1-PBS targets EIF2AK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16970

  Product name: Recombinant human EIF2AK1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 281-630 aa of BC006524

  Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 1
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC006524
• Gene Symbol: EIF2AK1
• Gene ID (NCBI): 27102
• RRID: AB_2882869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BQI3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.