EIF2C3-Specific Polyclonal antibody, PBS Only

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  WB analysis of SKOV-3 using 19692-1-AP (same clone as 19692-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19692-1-AP (same clone as 19692-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19692-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19692-1-AP (same clone as 19692-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 19692-1-AP (same clone as 19692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19692-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 19692-1-AP (same clone as 19692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19692-1-PBS in a different storage buffer formulation.

EIF2C3-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 19692-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

19692-1-PBS targets EIF2C3-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2C, 3
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_024852
• Gene Symbol: EIF2C3
• Gene ID (NCBI): 192669
• RRID: AB_2878597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9G7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EIF2C3, also named as AGO3, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi). EIF2C3 binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. EIF2C3 lacks endonuclease activity and does not appear to cleave target mRNAs. This antibody is specific to EIF2C3.