ELAVL4 Monoclonal antibody

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  WB analysis of U2OS using 67835-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67835-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67835-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67835-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67835-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 67835-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ELAVL4 antibody (67835-1-Ig, Clone: 1B1F5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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  FC experiment of U2OS using 67835-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ELAVL4 (67835-1-Ig, Clone:1B1F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ELAVL4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 67835-1-Ig

Clone No.1B1F5

Publications(3)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Synonyms

HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4

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Tested Applications

• Positive WB detected in: pig brain tissue, U2OS cells, SH-SY5Y cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, rat cerebellum tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

67835-1-Ig targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3678

  Product name: Recombinant human ELAVL4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC036071

  Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVGQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAITSLNGYRLGDRVLQVSFKTNKAHKS

• Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
• Calculated Molecular Weight: 380 aa, 42 kDa
• Observed Molecular Weight: 32-40 kDa
• GenBank Accession Number: BC036071
• Gene Symbol: ELAVL4
• Gene ID (NCBI): 1996
• RRID: AB_2918597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26378
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Int J Mol Sci; Flow Cytometric Analysis and Sorting of Murine Enteric Nervous System Cells: An Optimized Protocol; Authors - Faidra Karkala
mouse	CoIP	Mol Neurobiol; NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress, Neuronal Loss, and Glial Activation After Status Epilepticus; Authors - Xiaoying Gao
rat	WB,IF	Int J Mol Sci; The Antidepressant Effect of Resveratrol Is Related to Neuroplasticity Mediated by the ELAVL4-Bdnf mRNA Pathway; Authors - Hailong Ge