ENDOG Recombinant antibody, PBS Only

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  WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86122-1-RR (same clone as 86122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 86122-1-RR (same clone as 86122-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86122-1-RR (ENDOG antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86122-1-PBS in a different storage buffer formulation.

ENDOG Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86122-1-PBS

Clone No.250804D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EC:3.1.30.-, Endo G, Endonuclease G

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Product Information

86122-1-PBS targets ENDOG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ENDOG fusion protein Ag17739
• Full Name: endonuclease G
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC016351
• Gene Symbol: ENDOG
• Gene ID (NCBI): 2021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14249
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.