ENDOG Recombinant antibody

ENDOG Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86122-1-RR
Clone No.250804D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

EC:3.1.30.-, Endo G, Endonuclease G

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86122-1-RR targets ENDOG in applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ENDOG fusion protein Ag17739 Predict reactive species
Full Name endonuclease G
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC016351
Gene Symbol ENDOG
Gene ID (NCBI) 2021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.

Protocols

Product Specific Protocols
WB protocol for ENDOG antibody 86122-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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