ENDOG Recombinant monoclonal antibody

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  WB analysis using 86122-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86122-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86122-1-RR (ENDOG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 86122-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 86122-1-RR (ENDOG antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86122-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86122-1-RR against Human ENDOG were performed. The affinity constant is 0.659 nM.

ENDOG Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86122-1-RR

Clone No.250804D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.30.-, Endo G, Endonuclease G

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86122-1-RR targets ENDOG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag17739

  Product name: Recombinant human ENDOG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 63-297 aa of BC016351

  Sequence: ELAKYGLPGLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGKSYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNILARAGSLKAITAGSK

• Full Name: endonuclease G
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC016351
• Gene Symbol: ENDOG
• Gene ID (NCBI): 2021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14249
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.