Tested Applications
Positive WB detected in | NIH/3T3 cells, HeLa cells, mouse heart tissue, mouse liver tissue, rat liver tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86122-1-RR targets ENDOG in applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | ENDOG fusion protein Ag17739 Predict reactive species |
Full Name | endonuclease G |
Calculated Molecular Weight | 297 aa, 33 kDa |
Observed Molecular Weight | 25-30 kDa |
GenBank Accession Number | BC016351 |
Gene Symbol | ENDOG |
Gene ID (NCBI) | 2021 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14249 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Endonuclease G, also named as EndoG, is a mitochondrial protein. It's a nuclease which was first characterized in bovine heart mitochondrial extracts. It's involved in many cellular process, including apoptosis, paternal mitochondrial elimination and autophage (PMID:33473107). It is a nuclear encoded, sugar-non-specific (PMID:15066427) and well-conserved nuclease (PMID:17244531). It can be released from the mitochondria and translocated to the nucleus where it induces fragmentation of DNA, leading to apoptosis (PMID:11452314). EndoG is a 297-amino-acid long protein with a molecular weight of 30-35 kDa. There is a homodimer form with MW about 60-70 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for ENDOG antibody 86122-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |