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  • KD/KO Validated

NSE Monoclonal antibody

NSE Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig







Cat no : 66150-1-Ig


ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

Tested Applications

Positive WB detected inPC-12 cells, C6 cells, fetal human brain tissue, HEK-293 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, SH-SY5Y cells, U-251 cells, pig cerebellum tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue, human brain tissue, rat cerebellum tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66150-1-Ig targets NSE in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NSE fusion protein Ag19106
Full Name enolase 2 (gamma, neuronal)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC002745
Gene Symbol ENO2
Gene ID (NCBI) 2026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.


Product Specific Protocols
WB protocol for NSE antibody 66150-1-IgDownload protocol
IHC protocol for NSE antibody 66150-1-IgDownload protocol
IF protocol for NSE antibody 66150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang


Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.

Authors - Lin Lu

J Neurochem

Extracellular ATP and cAMP signaling promote Piezo2-dependent mechanical allodynia after trigeminal nerve compression injury.

Authors - Zhaoke Luo

CNS Neurosci Ther

Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.

Authors - Jing Deng


Effects of morphine preconditioning on TRPV1 sensitization and ERK1/2 phosphorylation induced by TGFβ1 in neurocytes.

Authors - Zhen-Xiao Ma

Biomed Res Int

In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI.

Authors - Tong Mu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Morgan (Verified Customer) (10-26-2021)

Overall it worked well. It looked a little dirty with some "extra" bands, but I'm not exactly sure why. The trend is still clear though.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: OV90 Ovarian Cancer Cells
NSE Antibody Western Blot validation (1:500 dilution) in OV90 Ovarian Cancer Cells (Cat no:66150-1-Ig)