Tested Applications
| Positive WB detected in | PC-12 cells, C6 cells, fetal human brain tissue, HEK-293 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, SH-SY5Y cells, U-251 cells, pig cerebellum tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, rat brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66150-1-Ig targets NSE in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19106 Product name: Recombinant human ENO2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2-300 aa of BC002745 Sequence: SIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDD Predict reactive species |
| Full Name | enolase 2 (gamma, neuronal) |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC002745 |
| Gene Symbol | NSE |
| Gene ID (NCBI) | 2026 |
| RRID | AB_2881546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09104-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NSE antibody 66150-1-Ig | Download protocol |
| IF protocol for NSE antibody 66150-1-Ig | Download protocol |
| IHC protocol for NSE antibody 66150-1-Ig | Download protocol |
| WB protocol for NSE antibody 66150-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NSE Monoclonal antibody (catalog number 66150-1-Ig) has 38 citations. These appear in journals including Nature Cell Biology, Nature Communications, Redox Biology, Cell Death & Disease, Cancer Letters, Apoptosis, Cell Reports, Oncogenesis, Journal of Pathology, and Scientific Reports, among others. Research fields span oncology (prostate, lung, pancreatic, colorectal, renal, and endometrial cancers), neuroscience (neuroinflammation, epilepsy, brain injury, neural differentiation), and cellular metabolism (glycolysis, enolase function). Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment | ||
Nat Commun Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice. | ||
J Exp Clin Cancer Res PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility | ||
Adv Sci (Weinh) Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer | ||
Cell Mol Biol Lett Trigeminal nerve root compression induced neuroinflammatory response promotes mechanical allodynia through the CGRP/SP-Piezo2 axis via Ca(2+) signaling. |
Reviews
What customers say
The single available review rates this antibody 4 out of 5 stars for Western Blot on OV90 ovarian cancer cells at a 1:500 dilution. The user reported that the antibody performed well overall and produced a clear trend, though they noted some "extra" bands that made the result look a bit dirty. Despite this minor issue, the reviewer found the antibody functional and useful for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Morgan (Verified Customer) (10-26-2021) | Overall it worked well. It looked a little dirty with some "extra" bands, but I'm not exactly sure why. The trend is still clear though.
 |
