|Positive WB detected in||PC-12 cells, C6 cells, fetal human brain tissue, HEK-293 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, SH-SY5Y cells, U-251 cells, pig cerebellum tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue|
|Positive IHC detected in||mouse brain tissue, human brain tissue, rat brain tissue, rat cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, mouse brain tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:5000|
|Immunofluorescence (IF)||IF : 1:50-1:400|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66150-1-Ig is NSE Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||NSE fusion protein Ag19106|
|Full Name||enolase 2 (gamma, neuronal)|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC002745|
|Gene ID (NCBI)||2026|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Morgan (Verified Customer) (10-26-2021)
Overall it worked well. It looked a little dirty with some "extra" bands, but I'm not exactly sure why. The trend is still clear though.