ENSA Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 14518-1-AP (same clone as 14518-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14518-1-AP (same clone as 14518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14518-1-AP (same clone as 14518-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 14518-1-AP (same clone as 14518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14518-1-AP (same clone as 14518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14518-1-AP (same clone as 14518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14518-1-AP (same clone as 14518-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENSA antibody (14518-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14518-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ARPP-19e, Alpha-endosulfine

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Product Information

14518-1-PBS targets ENSA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENSA fusion protein Ag5982
• Full Name: endosulfine alpha
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC004461
• Gene Symbol: ENSA
• Gene ID (NCBI): 2029
• RRID: AB_10838808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43768
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.