ENSA Polyclonal antibody

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14518-1-AP

Synonyms

ARPP-19e, Alpha-endosulfine

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Validation Data Gallery

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  WB analysis using 14518-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14518-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14518-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 14518-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 14518-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14518-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14518-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14518-1-AP (ENSA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, HeLa cells
• Positive IHC detected in: human small intestine tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14518-1-AP targets ENSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENSA fusion protein Ag5982
• Full Name: endosulfine alpha
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC004461
• Gene Symbol: ENSA
• Gene ID (NCBI): 2029
• RRID: AB_10838808
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.