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  • KD/KO Validated

EPCAM/CD326 Monoclonal antibody

EPCAM/CD326 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a









Cat no : 66316-1-Ig


CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

Tested Applications

Positive WB detected inHaCaT cells, A431 cells, T-47D cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, human colon cancer tissue, human stomach cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66316-1-Ig targets EPCAM/CD326 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen EPCAM/CD326 fusion protein Ag15393
Full Name epithelial cell adhesion molecule
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014785
Gene Symbol EPCAM
Gene ID (NCBI) 4072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)


Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 66316-1-IgDownload protocol
IHC protocol for EPCAM/CD326 antibody 66316-1-IgDownload protocol
IF protocol for EPCAM/CD326 antibody 66316-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

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Multi-modal imaging probe for EpCAM overexpressed in breast cancer.

Authors - Yaxue Dai
  • KD Validated

Cell Death Dis

Mutations in the coat complex II component SEC23B promote colorectal cancer metastasis.

Authors - Chunyuan Yang

Front Pharmacol

Photo-functionalized TiO2 film for facile immobilization of EpCAM antibodies and efficient enrichment of circulating tumor cells

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guorong (Verified Customer) (03-31-2022)

Seems not good for ARPE-19 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: ARPE-19
EPCAM/CD326 Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:66316-1-Ig)