EPCAM/CD326 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

EPCAM/CD326 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A2D5

Cat no : 66316-1-PBS

Synonyms

CD326, CO 17A, CO17 1A, EGP, EGP 2, EGP314, EGP34, EGP40, Ep CAM, EPCAM, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733 2, hEGP 2, hEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, M4S1, MIC18, MK 1, TACST 1, TACSTD1, TROP1

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Validation Data Gallery

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  WB analysis using 66316-1-Ig (same clone as 66316-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66316-1-Ig (same clone as 66316-1-PBS)

  WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66316-1-Ig (same clone as 66316-1-PBS)

  T-47D, MCF-7 and A431 cells were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66316-1-Ig (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EPCAM antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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  IF Staining of human stomach cancer using 66316-1-Ig (same clone as 66316-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using EPCAM/CD326 antibody (66316-1-Ig, Clone: 2A2D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66316-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66316-1-PBS targets EPCAM/CD326 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPCAM/CD326 fusion protein Ag15393
• Full Name: epithelial cell adhesion molecule
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014785
• Gene Symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)