EPLIN Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

  WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 16639-1-AP (same clone as 16639-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

  IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 16639-1-AP (same clone as 16639-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16639-1-AP (EPLIN antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using LIMA1 antibody 16639-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 16639-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

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Product Information

16639-1-PBS targets EPLIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPLIN fusion protein Ag9994
• Full Name: LIM domain and actin binding 1
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: BC001247
• Gene Symbol: EPLIN
• Gene ID (NCBI): 51474
• RRID: AB_2136657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHB6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)