EPOR Polyclonal antibody

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  WB analysis of HepG2 using 55308-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55308-1-AP (EPOR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55308-1-AP

  WB result of EPOR antibody (55308-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPOR transfected Jurkat cells.

• Featured Product
• KD/KO Validated

EPOR Polyclonal Antibody for WB, ELISA

Cat No. 55308-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

EPO R, EPO-R, erythropoietin receptor

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, K-562 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55308-1-AP targets EPOR in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: erythropoietin receptor
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 66 kDa, 78 kDa
• GenBank Accession Number: NM_000121
• Gene Symbol: EPOR
• Gene ID (NCBI): 2057
• RRID: AB_3086439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19235
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	PeerJ; RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA; Authors - Jin Fan