EPPK1 Recombinant antibody

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  WB analysis of L02 using 84344-2-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84344-2-RR (EPPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84344-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPPK1 antibody (84344-2-RR, Clone: 241633B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 84344-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 84344-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84344-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84344-2-RR against Human EPPK1 were performed. The affinity constant is 1.61 nM.

EPPK1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84344-2-RR

Clone No.241633B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84344-2-RR targets EPPK1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EPPK1 fusion protein Ag35749
• Full Name: epiplakin 1
• Calculated Molecular Weight: 555 kDa
• Observed Molecular Weight: 500-600 kDa
• GenBank Accession Number: NM_031308.3
• Gene Symbol: EPPK1
• Gene ID (NCBI): 83481
• RRID: AB_3671884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P58107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.