EPRS Monoclonal antibody
EPRS Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, rat, mouse
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
CloneNo.
1B7G2
Cat no : 67712-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-29 cells, HSC-T6 cells, NIH/3T3 cells, PC-12 cells, HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, Jurkat cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
67712-1-Ig targets EPRS in WB, IHC, IF,ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Reactivity | Human, rat, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | EPRS fusion protein Ag19184 |
Full Name | glutamyl-prolyl-tRNA synthetase |
Calculated molecular weight | 1512 aa, 171 kDa |
Observed molecular weight | 171 kDa |
GenBank accession number | BC126275 |
Gene symbol | EPRS |
Gene ID (NCBI) | 2058 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human EPRS is a 172 kDa, 1512 amino acid polypeptide consisting of three major domains. The N and C termini contain ERS and PRS catalytic domains, respectively, joined by a 300 amino acid linker containing three tandem WHEP-TRS (referred to as WHEP) domains. EPRS is a bifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. EPRS has a special role in GAIT-mediated translational control, as it is solely responsible for recognition and interaction with GAIT elements in target mRNAs. (PMID: 29576217, PMID: 22386318, PMID: 19647514)
Protocols
Product Specific Protocols | |
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WB protocol for EPRS antibody 67712-1-Ig | Download protocol |
IHC protocol for EPRS antibody 67712-1-Ig | Download protocol |
IF protocol for EPRS antibody 67712-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |