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- KD/KO Validated
ER Polyclonal antibody
ER Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IHC, IF, ELISA
Cat no : 21244-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MDA-MB-231 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 75 publications below|
|IHC||See 28 publications below|
|IF||See 14 publications below|
21244-1-AP targets ER in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ER fusion protein Ag15738|
|Full Name||estrogen receptor 1|
|Calculated molecular weight||595 aa, 66 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC128573|
|Gene ID (NCBI)||2099|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]
|Product Specific Protocols|
|WB protocol for ER antibody 21244-1-AP||Download protocol|
|IHC protocol for ER antibody 21244-1-AP||Download protocol|
|Click here to view our Standard Protocols|
PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII
J Hazard Mater
Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.
Betulinic acid alleviates zearalenone-induced uterine injury in mice
J Med Chem
Discovery of Thieno[2,3-e]indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability.
Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.
Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (05-07-2019)
There are multiple bands for this antibody.