ER Polyclonal antibody

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  WB analysis using 21244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 21244-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of NIH/3T3 using 21244-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 21244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ER Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21244-1-AP

Publications(138)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

ESR1, ER alpha, Era, ER-alpha, ERα

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 102 publications below
• IHC: See 38 publications below
• IF: See 23 publications below
• CoIP: See 2 publications below

Product Information

21244-1-AP targets ER in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ER fusion protein Ag15738
• Full Name: estrogen receptor 1
• Calculated Molecular Weight: 595 aa, 66 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC128573
• Gene Symbol: ER
• Gene ID (NCBI): 2099
• RRID: AB_11042600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03372
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Publications

Species	Application	Title
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
mouse	WB	J Biomed Sci; Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.; Authors - Chunfang Zhang
human	IHC	Theranostics; Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype; Authors - Zhi-Jie Gao
mouse	WB,IHC	J Hazard Mater; Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.; Authors - Dacheng Yang
human	WB	J Med Chem; Discovery of Thiochroman Derivatives as Potent, Oral Selective Estrogen Receptor Degraders and Antagonists for the Treatment of Endocrine-Resistant Breast Cancer; Authors - Dan Zhang
human	WB	J Environ Sci (China); The effects of 17β-trenbolone and bisphenol A on sexual behavior and social dominance via the hypothalamic-pituitary-gonadal axis in male mice; Authors - Xiang Zuo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (05-07-2019)

There are multiple bands for this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A/MCF7

ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)