ER Polyclonal antibody

• Featured Product
• KD/KO Validated

ER Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

100

Reviews (1)

Cat no : 21244-1-AP

Synonyms

ER, ER alpha, Era, ERα, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1

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Validation Data Gallery

• 

  WB analysis using 21244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21244-1-AP

  MCF-7 (ER+), MDA-MB-231 (ER-) cell lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21244-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of NIH/3T3 using 21244-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 21244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 21244-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 21244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP (ER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MDA-MB-231 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 75 publications below
• IHC: See 28 publications below
• IF: See 14 publications below

Product Information

21244-1-AP targets ER in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ER fusion protein Ag15738
• Full Name: estrogen receptor 1
• Calculated molecular weight: 595 aa, 66 kDa
• Observed molecular weight: 65-70 kDa
• GenBank accession number: BC128573
• Gene symbol: ESR1
• Gene ID (NCBI): 2099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Publications

Species	Application	Title
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
mouse	WB,IHC	J Hazard Mater; Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.; Authors - Dacheng Yang
mouse	WB	Environ Pollut; Betulinic acid alleviates zearalenone-induced uterine injury in mice; Authors - Chenglin Yang
human	WB	J Med Chem; Discovery of Thieno[2,3-e]indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability.; Authors - Zhengyu Lu
mouse	IHC	Sci Rep; Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.; Authors - Zhenqi Qin
		Sci Rep; Generation of exogenous germ cells in the ovaries of sterile NANOS3-null beef cattle.; Authors - Atsushi Ideta

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (05-07-2019)

There are multiple bands for this antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF10A/MCF7

ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)

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