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  • KD/KO Validated

ER Polyclonal antibody

ER Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21244-1-AP

Synonyms

ER, ER alpha, Era, ERα, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21244-1-AP targets ER in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ER fusion protein Ag15738
Full Name estrogen receptor 1
Calculated Molecular Weight 595 aa, 66 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC128573
Gene Symbol ER
Gene ID (NCBI) 2099
RRIDAB_11042600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Protocols

Product Specific Protocols
WB protocol for ER antibody 21244-1-APDownload protocol
IHC protocol for ER antibody 21244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII

Authors - Xavier Rambout
mouseWB

J Biomed Sci

Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.

Authors - Chunfang Zhang
mouseWB

J Hazard Mater

Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.

Authors - Dacheng Yang
mouseWB

Environ Pollut

Betulinic acid alleviates zearalenone-induced uterine injury in mice

Authors - Chenglin Yang
humanWB

J Med Chem

Discovery of Thieno[2,3-e]indazole Derivatives as Novel Oral Selective Estrogen Receptor Degraders with Highly Improved Antitumor Effect and Favorable Druggability.

Authors - Zhengyu Lu
mouse

Sci Rep

Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.

Authors - Zhenqi Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (05-07-2019)

There are multiple bands for this antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A/MCF7
ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)