• Featured Product
  • KD/KO Validated

EXOC1 Polyclonal antibody

EXOC1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11690-1-AP

Synonyms

BM 102, EXOC1, exocyst complex component 1, Exocyst complex component Sec3, SEC3, SEC3L1, SEC3P



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11690-1-AP targets EXOC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOC1 fusion protein Ag2303
Full Name exocyst complex component 1
Calculated molecular weight 894 aa, 102 kDa
Observed molecular weight 102 kDa
GenBank accession numberBC020650
Gene symbol EXOC1
Gene ID (NCBI) 55763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Protocols

Product Specific Protocols
WB protocol for EXOC1 antibody 11690-1-APDownload protocol
IHC protocol for EXOC1 antibody 11690-1-APDownload protocol
IP protocol for EXOC1 antibody 11690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IF

Sci Rep

Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics.

Authors - Sung Jin Park
humanWB,IF

Placenta

Exocyst complex protein expression in the human placenta.

Authors - I M Gonzalez
humanWB,IF

Cell Cycle

EXO1 overexpression is associated with poor prognosis of hepatocellular carcinoma patients.

Authors - Yaoyao Dai
  • KD Validated
ratWB,IF

Am J Physiol Endocrinol Metab

The exocyst complex regulates insulin-stimulated glucose uptake of skeletal muscle cells

Authors - Brent A Fujimoto
humanIF

Mol Biol Cell

Syntaxin 16 is a master recruitment factor for cytokinesis.

Authors - Neto Hélia H
humanWB,IP

Retrovirology

Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.

Authors - Mukerji Joya J