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  • KD/KO Validated

EXOC1 Polyclonal antibody

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 11690-1-AP

Synonyms

SEC3P, SEC3L1, SEC3, Exocyst complex component Sec3, Exocyst complex component 1



Tested Applications

Positive WB detected inrat brain tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11690-1-AP targets EXOC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOC1 fusion protein Ag2303
Full Name exocyst complex component 1
Calculated Molecular Weight 894 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC020650
Gene Symbol EXOC1
Gene ID (NCBI) 55763
RRIDAB_2231500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Protocols

Product Specific Protocols
WB protocol for EXOC1 antibody 11690-1-APDownload protocol
IHC protocol for EXOC1 antibody 11690-1-APDownload protocol
IF protocol for EXOC1 antibody 11690-1-APDownload protocol
IP protocol for EXOC1 antibody 11690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide conversion mechanism for exit from endosomes.

Authors - Katharina Ketel
  • KD Validated
humanWB,IF

Nat Nanotechnol

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Authors - Tanmoy Saha
  • KD Validated
mouseWB

J Cell Biol

The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.

Authors - Juan José Sáez
mouseIF

Elife

EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.

Authors - Yuki Osawa
humanIF

PLoS Pathog

Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.

Authors - Yuen-Yan Chang
humanWB,IP

Retrovirology

Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.

Authors - Mukerji Joya J