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  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 11178-1-AP


Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11178-1-AP targets EXOSC10 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag1666
Full Name exosome component 10
Calculated Molecular Weight 98 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.


Product Specific Protocols
WB protocol for EXOSC10 antibody 11178-1-APDownload protocol
IHC protocol for EXOSC10 antibody 11178-1-APDownload protocol
IF protocol for EXOSC10 antibody 11178-1-APDownload protocol
IP protocol for EXOSC10 antibody 11178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic

Cell Rep

Human Telomerase RNA Processing and Quality Control.

Authors - Chi-Kang Tseng
  • KD Validated


Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.

Authors - Pauline Antonie Ulmke
  • KO Validated


Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Roy (Verified Customer) (06-12-2024)

Did detect EXOSC10 by WB (1/1000 - overnight incubation at 4°C) in HeLa cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa

Tsimafei (Verified Customer) (12-17-2023)

Antibody detect Exosc10 (around 100 kDa on the image). However non-specific bands are observed in higher molecular weight area

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mESCs
EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11178-1-AP)