|Positive WB detected in||HeLa cells|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11178-1-AP targets EXOSC10 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EXOSC10 fusion protein Ag1666|
|Full Name||exosome component 10|
|Calculated molecular weight||98 kDa|
|GenBank accession number||BC039901|
|Gene ID (NCBI)||5394|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.
|Product Specific Protocols|
|WB protocol for EXOSC10 antibody 11178-1-AP||Download protocol|
|IHC protocol for EXOSC10 antibody 11178-1-AP||Download protocol|
|IF protocol for EXOSC10 antibody 11178-1-AP||Download protocol|
|IP protocol for EXOSC10 antibody 11178-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.
Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.
Human Telomerase RNA Processing and Quality Control.