EXOSC10 Polyclonal antibody

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  WB analysis of HeLa using 16731-1-AP

  WB result of EXOSC10 antibody (16731-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

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  WB analysis using 16731-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 16731-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16731-1-AP (EXOSC10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16731-1-AP

  IP result of anti-EXOSC10 (IP:16731-1-AP, 4ug; Detection:16731-1-AP 1:700) with HeLa cells lysate 1200ug.

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  IHC staining of mouse skin using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 16731-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16731-1-AP (EXOSC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16731-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC10 antibody (16731-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16731-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

16731-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC10 fusion protein Ag10189
• Full Name: exosome component 10
• Calculated Molecular Weight: 885 aa, 101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC073788
• Gene Symbol: EXOSC10
• Gene ID (NCBI): 5394
• RRID: AB_2101730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01780
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

Publications

Species	Application	Title
human	WB,IHC	PeerJ; EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification; Authors - Zhi-Yong Meng ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-17-2023)

Antibody recognise Exosc10 band and some other non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:16731-1-AP)