• Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 16731-1-AP


Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16731-1-AP targets EXOSC10 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC10 fusion protein Ag10189
Full Name exosome component 10
Calculated Molecular Weight 885 aa, 101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC073788
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.


Product Specific Protocols
WB protocol for EXOSC10 antibody 16731-1-APDownload protocol
IHC protocol for EXOSC10 antibody 16731-1-APDownload protocol
IF protocol for EXOSC10 antibody 16731-1-APDownload protocol
IP protocol for EXOSC10 antibody 16731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification

Authors - Zhi-Yong Meng
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tsimafei (Verified Customer) (12-17-2023)

Antibody recognise Exosc10 band and some other non-specific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mESCs
EXOSC10 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:16731-1-AP)