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EZH2 Monoclonal antibody

EZH2 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1F10A12

Cat no : 66476-1-Ig

Synonyms

Enhancer of zeste homolog 2, ENX 1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Sample-dependent, check data in validation data gallery

Product Information

66476-1-Ig targets EZH2 in WB, IHC, FC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated molecular weight 751 aa, 86 kDa
Observed molecular weight 85 kDa, 100 kDa
GenBank accession numberBC010858
Gene symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Protocols

Product Specific Protocols
WB protocol for EZH2 antibody 66476-1-IgDownload protocol
FC protocol for EZH2 antibody 66476-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Cell Dev Biol

Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter.

Authors - Yan Lu
  • KD Validated
humanWB

Cell Death Dis

Long noncoding RNA MEG3 regulates LATS2 by promoting the ubiquitination of EZH2 and inhibits proliferation and invasion in gallbladder cancer.

Authors - Longyang Jin