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EZH2 Monoclonal antibody

EZH2 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (1)







Cat no : 66476-1-Ig


Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, Jurkat cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells, A431 cells, PC-3 cells, DU145 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66476-1-Ig targets EZH2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated Molecular Weight 751 aa, 86 kDa
Observed Molecular Weight 90-102 kDa
GenBank Accession NumberBC010858
Gene Symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)


Product Specific Protocols
WB protocol for EZH2 antibody 66476-1-IgDownload protocol
FC protocol for EZH2 antibody 66476-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

Long noncoding RNA MEG3 regulates LATS2 by promoting the ubiquitination of EZH2 and inhibits proliferation and invasion in gallbladder cancer.

Authors - Longyang Jin

Front Cell Dev Biol

Exosome-Delivered LncHEIH Promotes Gastric Cancer Progression by Upregulating EZH2 and Stimulating Methylation of the GSDME Promoter.

Authors - Yan Lu
  • KD Validated

Front Cell Dev Biol

Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery

Authors - Nicholas Marano

Cancer Med

LncRNA SPRY4-IT1 facilitates cell proliferation and angiogenesis of glioma via the miR-101-3p/EZH2/VEGFA signaling axis

Authors - Ji Wang

Int Immunopharmacol

Celastrol attenuates diabetic nephropathy by upregulating SIRT1-mediated inhibition of EZH2related wnt/β-catenin signaling

Authors - Yuewen Tang

Bioact Mater

Engineered mesenchymal stem cell-derived small extracellular vesicles for diabetic retinopathy therapy through HIF-1α/EZH2/PGC-1α pathway

Authors - Fengtian Sun