Tissue Factor/CD142 Monoclonal antibody

• 

  WB analysis of A431 using 67056-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 67056-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67056-1-Ig (Tissue Factor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tissue Factor/CD142 Monoclonal Antibody for WB, ELISA

Cat No. 67056-1-Ig

Clone No.2E4A1

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

CD142, Coagulation factor III, F3, Tissue factor, 2E4A1

---

Tested Applications

• Positive WB detected in: A431 cells, human blood tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67056-1-Ig targets Tissue Factor/CD142 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Tissue Factor/CD142 fusion protein Ag27563
• Full Name: coagulation factor III (thromboplastin, tissue factor)
• Calculated Molecular Weight: 295 aa, 33 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC011029
• Gene Symbol: F3
• Gene ID (NCBI): 2152
• RRID: AB_2882367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13726
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Acta Pharmacol Sin; Urolithin A promotes atherosclerotic plaque stability by limiting inflammation and hypercholesteremia in Apolipoprotein E-deficient mice; Authors - Meng-Yun Xu
human	WB	Heliyon; Enhancement of the function of mesenchymal stem cells by using a GMP-grade three-dimensional hypoxic large-scale production system; Authors - Yiyao Qi