FABP5 Polyclonal antibody

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  WB analysis of A375 using 12348-1-AP

  WB result of FABP5 antibody (12348-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

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  WB analysis using 12348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12348-1-AP

  N/A

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  WB analysis of mouse skin using 12348-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12348-1-AP

  IP result of anti-FABP5 (IP:12348-1-AP, 4ug; Detection:12348-1-AP 1:4000) with A375 cells lysate 1520 ug.

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  IHC staining of mouse skin using 12348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12348-1-AP (FABP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 12348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12348-1-AP (FABP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FABP5 antibody (12348-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of HeLa using 12348-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug FABP5 Polyclonal antibody (12348-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

FABP5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12348-1-AP

Publications(43)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

PAFABP, Fatty acid-binding protein, epidermal, Fatty acid-binding protein 5, Fatty acid binding protein 5, Epidermal-type fatty acid-binding protein

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Tested Applications

• Positive WB detected in: A375 cells, Hepa1-6 cells, HepG2 cells, mouse skin tissue, WB result of FABP5 antibody (12348-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and FABP5 knockout Hepa1-6 cells., HeLa cells, A431 cells
• Positive IP detected in: A375 cells
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 35 publications below
• IHC: See 12 publications below
• IF: See 9 publications below
• CoIP: See 3 publications below

Product Information

12348-1-AP targets FABP5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP5 fusion protein Ag3005
• Full Name: fatty acid binding protein 5 (psoriasis-associated)
• Calculated Molecular Weight: 135 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC019385
• Gene Symbol: FABP5
• Gene ID (NCBI): 2171
• RRID: AB_2100341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01469
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publications

Species	Application	Title
mouse	CoIP	Nat Metab; Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis; Authors - Xiang Zhang
human,mouse	WB, CoIP	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
rat	WB	Phytomedicine; Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept; Authors - Tao Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (01-09-2023)

Robust immunoreactivity detected.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse nerve