FABP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

FABP5 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 12348-1-AP

Synonyms

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP

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Validation Data Gallery

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  WB analysis of A375 using 12348-1-AP

  WB result of FABP5 antibody (12348-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

• 

  WB analysis using 12348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 12348-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 12348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 12348-1-AP (FABP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 12348-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.20 ug/test Anti-Human FABP5 (12348-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, mouse skin tissue, HeLa cells, A431 cells
• Positive IF detected in: U2OS cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

12348-1-AP targets FABP5 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FABP5 fusion protein Ag3005
• Full Name: fatty acid binding protein 5 (psoriasis-associated)
• Calculated molecular weight: 135 aa, 15 kDa
• Observed molecular weight: 15 kDa
• GenBank accession number: BC019385
• Gene symbol: FABP5
• Gene ID (NCBI): 2171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publications

Species	Application	Title
human,mouse	WB, CoIP	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	IF	Antioxid Redox Signal; Dual inhibition of PI3K/Akt and mTOR by the Dietary Antioxidant Delphinidin Ameliorates Psoriatic Features In-vitro and in an Imiquimod-induced Psoriasis-like Disease in Mice.; Authors - Jean Christopher Chamcheu
human	WB	Cell Death Dis; The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death.; Authors - Ge Liu

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