FADD Polyclonal antibody

FADD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14906-1-AP

Synonyms

FADD, GIG3, MORT1, Protein FADD



Tested Applications

Positive WB detected inHT-1080 cells, mouse pancreas tissue, A549 cells, mouse bone marrow, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14906-1-AP targets FADD in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, sheep, monkey, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FADD fusion protein Ag6701
Full Name Fas (TNFRSF6)-associated via death domain
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-30 kDa
GenBank Accession NumberBC000334
Gene Symbol FADD
Gene ID (NCBI) 8772
RRIDAB_2100486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Protocols

Product Specific Protocols
WB protocol for FADD antibody 14906-1-APDownload protocol
IHC protocol for FADD antibody 14906-1-APDownload protocol
IF protocol for FADD antibody 14906-1-APDownload protocol
IP protocol for FADD antibody 14906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang
mouseWB

PLoS Biol

A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.

Authors - Yingying Zhang
humanWB

Eur J Med Chem

PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism

Authors - Hongyan Lin
ratWB,IHC

Cancers (Basel)

Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1

Authors - Tiantian Qiu
humanWB,CoIP

Cancer Biol Med

A causal variant rs3769823 in 2q33.1 involved in apoptosis pathway leading to a decreased risk of non-small cell lung cancer

Authors - Xu Zhang
ratWB

Cell Cycle

N-acetylserotonin protects PC12 cells from hydrogen peroxide induced damage through ROS mediated PI3K / AKT pathway.

Authors - Jihe Kang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Isha (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:800 dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: kidney