Validation Data Gallery
|Positive WB detected in||HT-1080 cells, mouse pancreas tissue, A549 cells, mouse bone marrow, HeLa cells, HepG2 cells, Jurkat cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HT-1080 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14906-1-AP targets FADD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, sheep, monkey, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FADD fusion protein Ag6701|
|Full Name||Fas (TNFRSF6)-associated via death domain|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||23-30 kDa|
|GenBank accession number||BC000334|
|Gene ID (NCBI)||8772|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)
|Product Specific Protocols|
|WB protocol for FADD antibody 14906-1-AP||Download protocol|
|IHC protocol for FADD antibody 14906-1-AP||Download protocol|
|IF protocol for FADD antibody 14906-1-AP||Download protocol|
|IP protocol for FADD antibody 14906-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isha (Verified Customer) (12-15-2021)
Got really good bands in kidney lysates at 1:800 dilution