FADD Polyclonal antibody, PBS Only

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  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 14906-1-AP (same clone as 14906-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14906-1-AP (same clone as 14906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, MORT1

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Product Information

14906-1-PBS targets FADD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FADD fusion protein Ag6701
• Full Name: Fas (TNFRSF6)-associated via death domain
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-30 kDa
• GenBank Accession Number: BC000334
• Gene Symbol: FADD
• Gene ID (NCBI): 8772
• RRID: AB_2100486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13158
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)