CoraLite® Plus 488-conjugated FADD Recombinant antibody

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  IF Staining of HeLa using CL488-84619-5

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FADD antibody (CL488-84619-5, Clone: 241976A11 ) at dilution of 1:200, CL594-Phalloidin (red).

FADD Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-84619-5

Clone No.241976A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241976A11, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, MORT1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84619-5 targets FADD in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FADD fusion protein Ag6701
• Full Name: Fas (TNFRSF6)-associated via death domain
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000334
• Gene Symbol: FADD
• Gene ID (NCBI): 8772
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13158
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)