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  • KD/KO Validated

FAK Polyclonal antibody

FAK Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12636-1-AP

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2



Tested Applications

Positive WB detected inMCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

12636-1-AP targets FAK in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAK fusion protein Ag3331
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene symbol PTK2
Gene ID (NCBI) 5747
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protocols

Product Specific Protocols
WB protocol for FAK antibody 12636-1-APDownload protocol
IHC protocol for FAK antibody 12636-1-APDownload protocol
IF protocol for FAK antibody 12636-1-APDownload protocol
IP protocol for FAK antibody 12636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA.

Authors - Ding Jie J
mouseWB

J Hazard Mater

Polystyrene microplastics induced male reproductive toxicity in mice.

Authors - Haibo Jin
humanWB

Oncogene

Circadian regulator NR1D2 regulates glioblastoma cell proliferation and motility.

Authors - Min Yu
humanWB,IHC

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang
humanWB

Cancer Lett

Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis.

Authors - Yue Li
humanWB

Mater Sci Eng C Mater Biol Appl

Mg-Fe layered double hydroxides modified titanium enhanced the adhesion of human gingival fibroblasts through regulation of local pH level.

Authors - Yijia Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nathalie (Verified Customer) (07-12-2022)

Good for ImmunoHistochemistry

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: Human Skin