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  • KD/KO Validated

FAK Polyclonal antibody

FAK Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12636-1-AP

Synonyms

FADK, FADK 1, FAK, FAK1, Focal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2



Tested Applications

Positive WB detected inMCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12636-1-AP targets FAK in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAK fusion protein Ag3331
Full Name PTK2 protein tyrosine kinase 2
Calculated molecular weight 1052 aa, 119 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC028733
Gene symbol PTK2
Gene ID (NCBI) 5747
RRIDAB_2173668
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protocols

Product Specific Protocols
WB protocol for FAK antibody 12636-1-APDownload protocol
IHC protocol for FAK antibody 12636-1-APDownload protocol
IF protocol for FAK antibody 12636-1-APDownload protocol
IP protocol for FAK antibody 12636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA.

Authors - Ding Jie J
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
mouseWB

J Hazard Mater

Polystyrene microplastics induced male reproductive toxicity in mice.

Authors - Haibo Jin
humanWB

Cell Rep

Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells

Authors - Yingqi Liu
humanWB

Oncogene

Circadian regulator NR1D2 regulates glioblastoma cell proliferation and motility.

Authors - Min Yu
humanWB,IHC

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nathalie (Verified Customer) (07-12-2022)

Good for ImmunoHistochemistry

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: Human Skin