FANCG Polyclonal antibody

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  WB analysis of Jurkat using 10215-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10215-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10215-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10215-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 10215-1-AP

  IP result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of HeLa using 10215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCG antibody (10215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FANCG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10215-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

10215-1-AP targets FANCG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0249

  Product name: Recombinant human FANCG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 125-348 aa of BC000032

  Sequence: VLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKH

• Full Name: Fanconi anemia, complementation group G
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC000032
• Gene Symbol: FANCG
• Gene ID (NCBI): 2189
• RRID: AB_2231541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15287
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	IF	J Cancer; Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma.; Authors - Yi Liu
human	WB	Clin Transl Oncol; Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study.; Authors - Yuzhong Cui
		Nat Commun; Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma; Authors - Wei Liu