FANCG Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 10215-1-AP (same clone as 10215-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 10215-1-AP (same clone as 10215-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10215-1-AP (same clone as 10215-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 10215-1-AP (same clone as 10215-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10215-1-AP (same clone as 10215-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10215-1-AP (FANCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 10215-1-AP (same clone as 10215-1-PBS)

  IP result of anti-FANCG (IP:10215-1-AP, 4ug; Detection:10215-1-AP 1:300) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10215-1-AP (same clone as 10215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCG antibody (10215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10215-1-PBS in a different storage buffer formulation.

FANCG Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 10215-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9

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Product Information

10215-1-PBS targets FANCG in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FANCG fusion protein Ag0249
• Full Name: Fanconi anemia, complementation group G
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC000032
• Gene Symbol: FANCG
• Gene ID (NCBI): 2189
• RRID: AB_2231541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15287
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).