FANCG Polyclonal antibody

FANCG Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 10215-1-AP

Synonyms

DNA repair protein XRCC9, FAG, FANCG, Fanconi anemia group G protein, Protein FACG, XRCC9



Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue
Positive IP detected inmouse testis tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10215-1-AP targets FANCG in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FANCG fusion protein Ag0249
Full Name Fanconi anemia, complementation group G
Calculated molecular weight 69 kDa
Observed molecular weight 65-68 kDa
GenBank accession numberBC000032
Gene symbol FANCG
Gene ID (NCBI) 2189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).

Protocols

Product Specific Protocols
WB protocol for FANCG antibody 10215-1-APDownload protocol
IF protocol for FANCG antibody 10215-1-APDownload protocol
IP protocol for FANCG antibody 10215-1-APDownload protocol
Standard Protocols
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