Tested Applications
| Positive WB detected in | Jurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10215-1-AP targets FANCG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0249 Product name: Recombinant human FANCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-348 aa of BC000032 Sequence: VLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKH Predict reactive species |
| Full Name | Fanconi anemia, complementation group G |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 65-68 kDa |
| GenBank Accession Number | BC000032 |
| Gene Symbol | FANCG |
| Gene ID (NCBI) | 2189 |
| RRID | AB_2231541 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15287 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FANCG antibody 10215-1-AP | Download protocol |
| IP protocol for FANCG antibody 10215-1-AP | Download protocol |
| WB protocol for FANCG antibody 10215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FANCG Polyclonal antibody (Cat# 10215-1-AP) has 4 total citations. These appear in Nature Communications, Science Advances, Journal of Cancer, and Clinical and Translational Oncology. The research fields span DNA repair mechanisms, Fanconi anemia pathway dysfunction, and cancer biology—including squamous cell carcinoma, colon cancer, ovarian carcinoma, and esophageal squamous cell carcinoma—covering topics such as glycolytic inhibition, radio sensitization, and tumor suppression.
| Species | Application | Title |
|---|---|---|
Nat Commun Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinoma | ||
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
J Cancer Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma. | ||
Clin Transl Oncol Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study. |
