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- KD/KO Validated
FAS/CD95 Monoclonal antibody
FAS/CD95 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1G7F7
Cat no : 60196-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
60196-1-Ig targets FAS/CD95 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, Zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | FAS/CD95 fusion protein Ag3718 |
| Full Name | Fas (TNF receptor superfamily, member 6) |
| Calculated molecular weight | 35-38 kDa |
| Observed molecular weight | 38-45 kDa |
| GenBank accession number | BC012479 |
| Gene symbol | FAS |
| Gene ID (NCBI) | 355 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FAS/CD95 antibody 60196-1-Ig | Download protocol |
| IHC protocol for FAS/CD95 antibody 60196-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.
| ||
Biochim Biophys Acta Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways. | ||
iScience Loss of Adipose Growth Hormone Receptor in Mice Enhances Local Fatty Acid Trapping and Impairs Brown Adipose Tissue Thermogenesis. | ||
Food Funct Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota. | ||
PLoS One The protective role of human ghrelin in sepsis: Restoration of CD4 T cell proliferation. | ||
Onco Targets Ther GRHL2 Upregulation Predicts a Poor Prognosis and Promotes the Resistance of Serous Ovarian Cancer to Cisplatin. |
