FAS/CD95 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FAS/CD95 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G7F7

Cat no : 60196-1-PBS

Synonyms

ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6

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Validation Data Gallery

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  WB analysis of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60196-1-Ig (same clone as 60196-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60196-1-Ig (same clone as 60196-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60196-1-Ig (same clone as 60196-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60196-1-Ig (FAS/CD95 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60196-1-Ig (same clone as 60196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60196-1-Ig (FAS/CD95 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 60196-1-Ig (same clone as 60196-1-PBS)

  1X10^6 Jurkat cells were stained with 0.2ug FAS/CD95 antibody (60196-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60196-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60196-1-PBS targets FAS/CD95 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FAS/CD95 fusion protein Ag3718
• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 35-38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC012479
• Gene Symbol: FAS
• Gene ID (NCBI): 355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.