- Featured Product
- KD/KO Validated
FASN Polyclonal antibody
FASN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
139
Cat no : 10624-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HT-29 cells, HepG2 cells, mouse liver tissue, SH-SY5Y cells, rat liver tissue, L02 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HepG2 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 127 publications below |
IHC | See 21 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
Product Information
10624-2-AP targets FASN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, duck, mosue, pig, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FASN fusion protein Ag0975 |
Full Name | fatty acid synthase |
Calculated molecular weight | 272 kDa |
Observed molecular weight | 250-272 kDa |
GenBank accession number | BC007909 |
Gene symbol | FASN |
Gene ID (NCBI) | 2194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for FASN antibody 10624-2-AP | Download protocol |
IHC protocol for FASN antibody 10624-2-AP | Download protocol |
IF protocol for FASN antibody 10624-2-AP | Download protocol |
IP protocol for FASN antibody 10624-2-AP | Download protocol |
FC protocol for FASN antibody 10624-2-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Sci Transl Med A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques. | ||
Nat Chem Biol Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues. | ||
Hepatology Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH K (Verified Customer) (12-20-2020) | I got good results with this Ab for WB at (1:1000) and IHC (1:200)
|
FH Iram (Verified Customer) (09-18-2020) | FASN antibody gives a very clean bands.Excellent antibody
|