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FASN Polyclonal antibody

FASN Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (7)


WB, IP, IF, IHC, CoIP, ELISA, FC (Intra)



Cat no : 10624-2-AP


FAS, FASN, fatty acid synthase, OA 519, SDR27X1

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, mouse brown adipose tissue, rat brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10624-2-AP targets FASN in WB, IP, IF, IHC, CoIP, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, zebrafish, pig, duck, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FASN fusion protein Ag0975
Full Name fatty acid synthase
Calculated Molecular Weight 272 kDa
Observed Molecular Weight 250-272 kDa
GenBank Accession NumberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.


Product Specific Protocols
WB protocol for FASN antibody 10624-2-APDownload protocol
IHC protocol for FASN antibody 10624-2-APDownload protocol
IF protocol for FASN antibody 10624-2-APDownload protocol
IP protocol for FASN antibody 10624-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan

Nat Commun

A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice

Authors - Yichao Lu

Sci Transl Med

A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques.

Authors - Xiao-Jing Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


K (Verified Customer) (12-20-2020)

I got good results with this Ab for WB at (1:1000) and IHC (1:200)

  • Applications: Western Blot, Immunohistochemistry
  • Cell Tissue Type: mouse liver tissues and rat liver

Iram (Verified Customer) (09-18-2020)

FASN antibody gives a very clean bands.Excellent antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer