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FASN Polyclonal antibody
FASN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10624-2-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|human breast cancer tissue, mouse brown adipose tissue, rat brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10624-2-AP targets FASN in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, goat, chicken, sheep, zebrafish, pig, duck, bovine
|Host / Isotype
|Rabbit / IgG
|FASN fusion protein Ag0975
|fatty acid synthase
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
|Product Specific Protocols
|WB protocol for FASN antibody 10624-2-AP
|IHC protocol for FASN antibody 10624-2-AP
|IF protocol for FASN antibody 10624-2-AP
|IP protocol for FASN antibody 10624-2-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (12-20-2020)
I got good results with this Ab for WB at (1:1000) and IHC (1:200)
Iram (Verified Customer) (09-18-2020)
FASN antibody gives a very clean bands.Excellent antibody