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- KD/KO Validated
FASN Polyclonal antibody
FASN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (5)
WB, IP, IHC, IF, FC, ELISA
Cat no : 10624-2-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HT-29 cells, HepG2 cells, mouse liver tissue, SH-SY5Y cells, rat liver tissue, L02 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 127 publications below|
|IHC||See 21 publications below|
|IF||See 5 publications below|
|IP||See 1 publications below|
10624-2-AP targets FASN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, duck, mosue, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FASN fusion protein Ag0975|
|Full Name||fatty acid synthase|
|Calculated molecular weight||272 kDa|
|Observed molecular weight||250-272 kDa|
|GenBank accession number||BC007909|
|Gene ID (NCBI)||2194|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.
|Product Specific Protocols|
|WB protocol for FASN antibody 10624-2-AP||Download protocol|
|IHC protocol for FASN antibody 10624-2-AP||Download protocol|
|IF protocol for FASN antibody 10624-2-AP||Download protocol|
|IP protocol for FASN antibody 10624-2-AP||Download protocol|
|FC protocol for FASN antibody 10624-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.
Sci Transl Med
A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques.
Nat Chem Biol
Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues.
Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.
Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (12-20-2020)
I got good results with this Ab for WB at (1:1000) and IHC (1:200)
Iram (Verified Customer) (09-18-2020)
FASN antibody gives a very clean bands.Excellent antibody