|Positive WB detected in||mouse liver tissue|
|Positive IF detected in||A549 cells, HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
11531-1-AP targets FBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FBF1 fusion protein Ag2106|
|Full Name||Fas (TNFRSF6) binding factor 1|
|Calculated molecular weight||1133 aa, 125 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC023549|
|Gene ID (NCBI)||85302|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.
A distal centriolar protein network controls organelle maturation and asymmetry.
TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.
Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.