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FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11531-1-AP

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863



Tested Applications

Positive WB detected inmouse liver tissue
Positive IF detected inA549 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11531-1-AP targets FBF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBF1 fusion protein Ag2106
Full Name Fas (TNFRSF6) binding factor 1
Calculated molecular weight1133 aa, 125 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC023549
Gene symbol FBF1
Gene ID (NCBI) 85302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protocols

Product Specific Protocols
WB protocol for FBF1 antibody 11531-1-APDownload protocol
IF protocol for FBF1 antibody 11531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanIF

Nat Commun

Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.

Authors - Kai He
humanIF

Nat Commun

A distal centriolar protein network controls organelle maturation and asymmetry.

Authors - Lei Wang
humanIF,WB

Nat Commun

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Authors - Hao Yan
humanIF

Nat Commun

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Authors - Qingwen Xu
human,mouseWB,IF

Nat Commun

Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.

Authors - Wei Qing Q
  • KD Validated