|Positive WB detected in||mouse liver tissue, COLO 320 cells, L02 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11531-1-AP targets FBF1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FBF1 fusion protein Ag2106|
|Full Name||Fas (TNFRSF6) binding factor 1|
|Calculated molecular weight||830 aa, 90 kDa|
|Observed molecular weight||130 kDa, 90 kDa, 70 kDa|
|GenBank accession number||BC023549|
|Gene ID (NCBI)||85302|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.
Cep128 associates with Odf2 to form the subdistal appendage of the centriole.
Albatross/FBF1 contributes to both centriole duplication and centrosome separation.
Fbf1 regulates mouse oocyte meiosis by influencing Plk1.
Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.
J Biol Chem
The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.