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  • KD/KO Validated

FBF1 Polyclonal antibody

FBF1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 11531-1-AP

Synonyms

Alb, albatross, Fas (TNFRSF6) binding factor 1, FBF 1, FBF1, FBF1 Ens, HCG1989313, isoform CRA_b, KIAA1863



Tested Applications

Positive WB detected inmouse liver tissue, COLO 320 cells, L02 cells
Positive IP detected inmouse liver tissue
Positive IF detected inA549 cells, HUVEC cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

11531-1-AP targets FBF1 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBF1 fusion protein Ag2106
Full Name Fas (TNFRSF6) binding factor 1
Calculated molecular weight 830 aa, 90 kDa
Observed molecular weight 130 kDa, 90 kDa, 70 kDa
GenBank accession numberBC023549
Gene symbol FBF1
Gene ID (NCBI) 85302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBF1, also named as ALB and KIAA1863, is keratin-binding protein required for epithelial cell polarization. It is involved in apical junction complex (AJC) assembly via its interaction with PARD3. FBF1 is required for ciliogenesis. FBF1 has six isoforms with MW 125-127 kDa, 99 kDa, 69 kDa and 55 kDa. This antibody is induced by N-terminal (20-347aa) of FBF1. It recognizes all the six isoforms.

Protocols

Product Specific Protocols
WB protocol for FBF1 antibody 11531-1-APDownload protocol
IF protocol for FBF1 antibody 11531-1-APDownload protocol
IP protocol for FBF1 antibody 11531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Genes Cells

Cep128 associates with Odf2 to form the subdistal appendage of the centriole.

Authors - Hiroka Kashihara
mouseWB,IF

Genes Cells

Albatross/FBF1 contributes to both centriole duplication and centrosome separation.

Authors - Akihito Inoko
  • KD Validated
mouseWB,IF

Theriogenology

Fbf1 regulates mouse oocyte meiosis by influencing Plk1.

Authors - Ying Xu
  • KD Validated
humanIF

Biophys J

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Authors - Rueyhung Roc Weng
humanWB

J Biol Chem

The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Authors - Adrian Cuenca
humanIF

Genetics

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Authors - Anna Kazatskaya